Association of EBV and HPV co-infection with the development of cervical cancer in ethnic Uyghur women
Association of EBV and HPV co-infection with the development of cervical cancer in ethnic Uyghur women
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维吾尔族妇女EBV和HPV合并感染与宫颈癌发生的关系
DOI：
10.12892/ejgo2701.2015
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Abudula, A.
中科院分区：
文献类型：
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作者：
Abudoukadeer, A.;Niyazi, M.;Abudula, A.
目的：研究EB病毒（EBV）和人乳头瘤病毒（HPV）感染在宫颈癌发生发展中的作用。材料与方法：我们收集了178例患有宫颈炎、宫颈上皮内瘤变（CIN I、CIN II-III）和宫颈鳞状细胞癌（CSCC）的维吾尔族妇女的宫颈组织标本。通过组织 DNA 的 PCR 检测 EBV-和 HPV-DNA。通过免疫组织化学检查EBV蛋白表达。结果：HPV-DNA 在宫颈炎、CIN I、CIN II-III 和宫颈癌病例中可检测到，分别为 2.5%、12.5%、68.0% 和 96.4%。对于 EBV-DNA，这些数字分别是 0、3.1、28.0 和 69.6%。宫颈炎组、CIN II-III 组和癌症组之间的 HPV 和 EBV 阳性率存在显着差异 (p < 0.05)。进一步分析发现，宫颈病变的发病机制不仅伴随着HPV或EBV-DNA单独感染率的逐渐升高，而且还伴随着HPV-EBV双重感染率的逐渐升高（r = 0.46；p < 0.01）。 EBV-DNA 阳性病例中 89.7%（34/39）EBV 蛋白表达呈阳性，EBV-DNA 阴性病例中 6%（1/17）EBV 蛋白表达呈阳性。结论：宫颈癌的发生、发展可能与HPV和EBV双重感染密切相关。
Objective: Study on the role of Epstein-Barr virus (EBV) and human papillomavirus (HPV) infection in the development of cervical cancer. Materials and Methods: We collected 178 cases of cervical tissue specimens of Uyghur women with cervicitis, cervical intraepithelial neoplasia (CIN I, CIN II-III), and cervical squamous cell carcinoma (CSCC). EBV- and HPV-DNA were detected by PCR of tissue DNA. EBV protein expression was checked by immunohistochemistry. Results: HPV-DNA was detectable in 2.5, 12.5, 68.0, and 96.4% of cases of cervicitis, CIN I, CIN II- III, and cervical cancer, respectively. For EBV-DNA, these numbers were 0, 3.1, 28.0, and 69.6%. There was a significant difference between the groups of cervicitis, CIN II-III, and cancer with respect to both HPV and EBV positivity rates (p < 0.05). Further analysis indicated that cervical lesion pathogenesis was not only accompanied by a gradually increasing rate of HPV or EBV-DNA alone, but also by an increasing rate of HPV-EBV dual infection (r = 0.46; p < 0.01). EBV protein expression was positive in 89.7% of EBV-DNA positive cases (34/39) and 6% of EBV-DNA negative cases (1/17). Conclusion: Cervical cancer development and progression may be closely associated with the dual-infection by HPV and EBV.