Variability in motility characteristics among marine bacteria
Variability in motility characteristics among marine bacteria
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DOI：
10.3354/ame028229
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发表时间：
2002-07-25
影响因子：
1.4
通讯作者：
Hagström, Å
中科院分区：
文献类型：
--
作者：
Johansen, JE;Pinhassi, J;Hagström, Å
使用相差显微镜结合数字图像分析研究了广泛分布的海洋细菌的系统发育多样性集合的运动特征。通过对 16S rDNA 进行测序，鉴定出 38 种分离细菌，本研究还包括另外 46 种未鉴定的细菌。鉴定出的细菌是γ-变形菌门（例如弧菌和假交替单胞菌）、α-变形菌门（例如玫瑰杆菌和柄杆菌）、噬细胞菌群成员和革兰氏阳性菌株。在 Zobell 培养基中生长的研究细菌的平均游泳速度范围为 11 至 38 mum s(-1)，大多数细菌的平均游泳速度为 15 至 25 mum s(-1)。最大速度达到 75 mum s(-1)。加速度范围为 80 至 189 mum s(-2)，平均为 121 mum s(-2)。平均运行时间范围在 0.105 到 0.323 秒之间。发现生长速度和平均游泳速度之间存在显着的正相关关系。总共 70% 的细菌分离株表现出大于 150 度（范围 123 至 180 度）的转动角度，这表明游泳方向反转是海洋环境中细菌的重要策略，这与肠道细菌表现出的有偏随机游走相反。
Motility characteristics of a phylogenetically diverse collection of widespread marine bacteria were investigated using phase contrast microscopy in combination with digital image analysis. Thirty-eight isolated bacteria were identified by sequencing the 16S rDNA and an additional 46 unidentified isolates were included in this study. The identified bacteria were gamma-Proteobacteria (e.g. Vibrio and Pseudoalteromonas), alpha-Proteobacteria (e.g. Roseobacter and Caulobacter), members of the Cytophaga group and a Gram-positive strain. The mean swimming speed of the investigated bacteria growing in Zobell medium ranged from 11 to 38 mum s(-1), with a majority of the bacteria having a mean speed of 15 to 25 mum s(-1). Maximum speeds reached 75 mum s(-1). Acceleration ranged from 80 to 189 mum s(-2) with an average of 121 mum s(-2). Mean run times ranged between 0.105 and 0.323 s. A significant positive correlation between growth rate and mean swimming speed was found. A total of 70% of the bacterial isolates showed a turn angle larger than 150degrees (range 123 to 180degrees), demonstrating that reversal of swimming direction is an important strategy for bacteria in the marine environment, in contrast to the biased random walk exhibited by enteric bacteria.