In utero fate mapping reveals distinct migratory pathways and fates of neurons born in the mammalian basal forebrain.
In utero fate mapping reveals distinct migratory pathways and fates of neurons born in the mammalian basal forebrain.
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发表时间：
2001-10
期刊：
影响因子：
4.6
通讯作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
中科院分区：
文献类型：
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作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
最近的研究表明，神经元出生在发展中的基底前脑迁移长距离垂直放射状胶质细胞和许多这些细胞到达发展中的新皮层。然而，这种形式的切线迁移，还没有在体内得到证实，和出生后的大脑中这些神经元的起源，迁移途径和最终目的地的网站还没有完全理解。利用超声引导的子宫内移植，我们绘制了13.5天龄小鼠胚胎中外侧和内侧神经节隆起（LGE和MGE）细胞的迁移途径和命运。我们证明，LGE和MGE细胞迁移沿着不同的路线，以填充不同的区域在发育中的大脑。我们发现，LGE细胞迁移腹侧和前部，并引起在纹状体，脑桥核和嗅结节，颗粒和球周细胞在嗅球中的突出中型多刺神经元。相比之下，我们表明，MGE是一个主要来源的神经元迁移背侧和入侵的发展中的新皮层。MGE细胞迁移到新皮层通过新皮层脑室下区和分化成短暂的软膜下颗粒神经元的边缘区和成一个稳定的人口GABA，小白蛋白或生长抑素表达的中间神经元在整个皮质板。
Recent studies suggest that neurons born in the developing basal forebrain migrate long distances perpendicularly to radial glia and that many of these cells reach the developing neocortex. This form of tangential migration, however, has not been demonstrated in vivo, and the sites of origin, pathways of migration and final destinations of these neurons in the postnatal brain are not fully understood. Using ultrasound-guided transplantation in utero, we have mapped the migratory pathways and fates of cells born in the lateral and medial ganglionic eminences (LGE and MGE) in 13.5-day-old mouse embryos. We demonstrate that LGE and MGE cells migrate along different routes to populate distinct regions in the developing brain. We show that LGE cells migrate ventrally and anteriorly, and give rise to the projecting medium spiny neurons in the striatum, nucleus accumbens and olfactory tubercle, and to granule and periglomerular cells in the olfactory bulb. By contrast, we show that the MGE is a major source of neurons migrating dorsally and invading the developing neocortex. MGE cells migrate into the neocortex via the neocortical subventricular zone and differentiate into the transient subpial granule neurons in the marginal zone and into a stable population of GABA-, parvalbumin- or somatostatin-expressing interneurons throughout the cortical plate.