Human umbilical cord blood cells improve cardiac function after myocardial infarction
Human umbilical cord blood cells improve cardiac function after myocardial infarction
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DOI：
10.1016/j.bbrc.2004.12.044
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发表时间：
2005-02-11
影响因子：
3.1
通讯作者：
Takamoto, S
中科院分区：
文献类型：
--
作者：
Hirata, Y;Sata, M;Takamoto, S
人脐带血（UCB）含有丰富的未成熟干/祖细胞，临床上已被用作骨髓移植的替代品。此外，与侵入性骨髓抽吸相比，脐带血可以非侵入性地获得。我们研究了人脐血CD 34（+）细胞改善心肌梗死后心功能的潜力。通过结扎Wistar大鼠左冠状动脉诱导心肌梗死。在诱导心肌梗死后，将2 × 10（5）个人UCB CD 34（+）细胞或等效的无细胞培养基注射到大鼠的损伤心肌中。与对照组相比，CD 34（+）细胞移植显著改善了心室功能。人CD 34、CD 45和PECAM-1的免疫荧光染色显示心肌中存活的细胞。我们的研究结果表明，移植的人类细胞存活，并改善心肌梗死后的心脏功能。这些结果可能表明脐血CD 34（+）细胞在心肌梗死中的作用。(C)2004年爱思唯尔公司All rights reserved.
Human umbilical cord blood (UCB) contains an abundance of immature stem/progenitor cells and has been clinically used as an alternative to bone marrow transplantation. In addition, cord blood can-be obtained non-invasively, in contrast to invasive bone marrow aspiration. We investigated the potential of human UCB CD34(+) cells to improve cardiac function following myocardial infarction. Myocardial infarction was induced in Wistar rats by ligation of the left coronary artery. Either 2 x 10(5) human UCB CD34(+) cells or equivalent cell-free medium was injected into the injured myocardium of the rats following induction of myocardial infarction. CD34(+) cell transplantation significantly improved ventricular function as compared to the control group. Immunofluorescence staining for human CD34, CD45, and PECAM-1 revealed surviving cells in the myocardium. Our findings suggest that transplanted human cells survived and improved cardiac function following myocardial infarction. These results may show the usefulness of UCB CD34(+) cells for myocardial infarction. (C) 2004 Elsevier Inc. All rights reserved.