Breeding of flocculent industrial alcohol yeast strains by self-cloning of the flocculation gene FLO1 and repeated-batch fermentation by transformants
Breeding of flocculent industrial alcohol yeast strains by self-cloning of the flocculation gene FLO1 and repeated-batch fermentation by transformants
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DOI：
10.2323/jgam.44.347
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发表时间：
1998-10-01
影响因子：
1.2
通讯作者：
Takagi, M
中科院分区：
文献类型：
--
作者：
Ishida-Fujii, K;Goto, S;Takagi, M
通过在URA 3位点引入功能性FLO 1基因，将非絮凝性工业多倍体酵母菌Saccharomycescerevisiae 396-9-6V转化为絮凝性酵母菌。所获得的絮凝菌株FSC 27是所谓的自克隆菌株，不具有细菌DNA。FSC 27细胞易于从发酵醪中回收利用，不需要任何物理能量，发酵速度略低于396-9-6V，但酒精浓度高达396-9-6V。在FSC 27（FSCU-L18）中加入尿嘧啶或重新引入URA 3后，发酵速率和生长速率均有所提高，乙醇浓度也高于出发菌株。FSCU-L18在10个循环的重复分批发酵试验中观察到稳定的絮凝和较高的乙醇产量。
A nonflocculent industrial polyploid yeast strain, Saccharomyces cerevisiae 396-9-6V, was converted to a flocculent one by introducing a functional FLO1 gene at the URA3 locus. The flocculent strain FSC27 obtained was a so-called self-cloned strain, having no bacterial DNA. FSC27 cells could be easily recovered for reuse from fermentation mash without any physical energy, The strain produced a concentration of alcohol as high as 396-9-6V, although the fermentation rate of FSC27 was slightly lower than that of 396-9-6V. When uracil was added to the medium or when URA3 was reintroduced into FSC27 (named FSCU-L18), the fermentation rate and the growth rate increased, and the ethanol concentration produced was higher than that produced by the parent strain, The stable flocculation and high ethanol productivity were observed by using FSCU-L18 during 10 cycles of repeated-batch fermentation test.