Theiler's virus infection in nude mice: viral RNA in vascular endothelial cells.
Theiler's virus infection in nude mice: viral RNA in vascular endothelial cells.
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裸鼠的泰勒病毒感染：血管内皮细胞中的病毒RNA。
DOI：
10.1128/jvi.62.10.3589-3596.1988
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发表时间：
1988
影响因子：
5.4
通讯作者：
Fujinami,RS
中科院分区：
文献类型：
--
作者：
Zurbriggen,A;Fujinami,RS
用泰勒氏鼠脑脊髓炎病毒感染无胸腺（nu/nu）小鼠可导致类似脊髓灰质炎的急性脑炎。免疫组织化学和原位杂交被用来描绘病毒蛋白质和RNA的存在下，在与丹尼尔斯株泰勒病毒感染的裸鼠的神经系统。该系统允许在缺乏有效免疫应答的情况下分析病毒感染。免疫组化显示，病毒抗原存在于神经元和神经胶质细胞的突起和胞体中。除了这些细胞类型中存在病毒抗原外，通过原位杂交，还在这些感染小鼠的大脑和脊髓中与血管内皮相关的细胞中发现了泰勒病毒RNA。Theiler病毒RNA阳性内皮细胞不仅在原发性病变附近观察到，而且在中枢神经系统正常外观区域的可证实病变之外也观察到。早期的研究表明这种病毒在轴突内传播（M。C. Dal Canto和H. L.利普顿，美国J.病理学106：20-29，1982）。我们的发现与这一模型一致，但也表明了病毒在中枢神经系统内传播的另一种机制，即，通过感染血管细胞和穿过血脑屏障。最后，在Theiler小鼠脑脊髓炎病毒感染后，不仅胶质细胞而且内皮细胞在其表面上表达主要组织相容性复合物II类（Ia）抗原（M.罗德里格斯，M。L. Pierce和E. A. 138：3438-3442，1987）。我们对Theiler病毒感染的内皮样细胞的证明可能解释了病毒产物在刺激抗原呈递中的相互作用。
Infection of athymic (nu/nu) mice with Theiler's murine encephalomyelitis virus results in an acute encephalitis which resembles poliomyelitis. Immunohistochemistry and in situ hybridization were used to delineate the presence of viral proteins and RNA in the nervous systems of nude mice infected with the Daniels strain of Theiler's virus. This system permits the analysis of a viral infection in the absence of an effective immune response. By immunohistochemistry, viral antigen was found in the processes and cell bodies of neurons and glial cells. Besides the presence of viral antigen in these cell types, by in situ hybridization, Theiler's virus RNA was also found in cells associated with vascular endothelium in the brains and spinal cords of these infected mice. Theiler's virus RNA-positive endothelial cells were observed not only near the primary lesions but also away from demonstrable lesions in normal-appearing regions in the central nervous system. Earlier work had suggested an intra-axonal dissemination for this virus (M. C. Dal Canto and H. L. Lipton, Am. J. Pathol. 106:20-29, 1982). Our findings are consistent with this model but also suggest an additional mechanism for virus spread within the central nervous system, i.e., by infecting vascular cells and crossing the blood-brain barrier. Lastly, after Theiler's murine encephalomyelitis virus infection, not only glial cells but also endothelial cells express major histocompatibility complex class II (la) antigen on their surface (M. Rodriguez, M. L. Pierce, and E. A. Howie, J. Immunol. 138:3438-3442, 1987). Our demonstration of Theiler's virus-infected endotheliumlike cells may explain interactions of virus products in stimulating antigen presentation.