Host-guest interaction of Hoechst 34580 and Cucurbit[7]uril.
Host-guest interaction of Hoechst 34580 and Cucurbit[7]uril.
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DOI：
10.1002/cphc.201100495
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Wanhua Lei;Qianxiong Zhou;Guoyu Jiang;Yuan-jun Hou;Bao-wen Zhang;Xuexin Cheng;Xuesong Wang
中科院分区：
文献类型：
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作者：
Wanhua Lei;Qianxiong Zhou;Guoyu Jiang;Yuan-jun Hou;Bao-wen Zhang;Xuexin Cheng;Xuesong Wang
为了通过荧光成像跟踪核动力学过程，核染色剂应该是高荧光的，耐光漂白的，并且对核过程惰性。Hoechst家族的核染色剂，如Hoechst 34580，仅在与DNA结合的沟上显示明亮的荧光，因此可能干扰由其他刺激诱导的核动力学过程的可视化。本文研究了Hoechst 34580与七元瓜环（CB 7）在水溶液中的相互作用。通过（1）H NMR、吸收光谱、荧光光谱、MALDI-TOF MS和分子模拟证实了CB 7-Hoechst 34580包合物在水中的化学计量比为2：1，在磷酸盐缓冲盐水（PBS）溶液（pH 7.0）中的化学计量比为1：1。与Hoechst 34580相比，该包合物具有吸收红移、荧光增强、光稳定性提高、DNA结合亲和力减弱、穿透细胞核能力相当、核染色能力增强等特点，为葫芦脲在荧光成像中的应用开辟了新的途径。
To track nuclear dynamic processes by fluorescence imaging, nuclear stains should be highly fluorescent, resistant to photobleaching, and inert to nuclear processes. The nuclear stains of the Hoechst family, such as Hoechst 34580, show bright fluorescence only on groove binding to DNA, and therefore may interfere with visualization of nuclear dynamic processes induced by other stimuli. We study host-guest interactions between Hoechst 34580 and Cucurbit[7]uril (CB7) in aqueous solutions. The formation of CB7-Hoechst 34580 inclusion complexes with stoichiometry of 2:1 in water and 1:1 in phosphate-buffered saline (PBS) solution (pH 7.0) is confirmed by (1)H NMR, absorption spectra, fluorescence spectra, MALDI-TOF MS, and molecular modeling. Compared to Hoechst 34580, the inclusion complex exhibits redshifted absorption, intensified fluorescence, improved photostability, weakened DNA binding affinity, comparable ability to penetrate cell nuclei, and better nuclear-staining capability, and thus a new avenue for the application of cucurbituril in fluorescence imaging is opened.