[Biological characteristics of an Hog1 MAPK homologous gene FoHog1 knock-out mutant of Fusarium oxysporum f. sp. cubense].
[Biological characteristics of an Hog1 MAPK homologous gene FoHog1 knock-out mutant of Fusarium oxysporum f. sp. cubense].
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发表时间：
2014-11
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通讯作者：
C. Mao;Pingya Chen;Qingdon Dai;Laying Yang;Junsheng Huang
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作者：
C. Mao;Pingya Chen;Qingdon Dai;Laying Yang;Junsheng Huang
目的从尖孢镰刀菌中克隆丝裂原活化蛋白激酶（MAPK）基因FoHog 1。sp. cubense进行了初步研究，并验证了其功能。方法采用PCR和RT-PCR方法扩增FoHog1基因，并进行生物信息学分析。利用PEG介导的原生质体转化法，获得FoHog1基因缺失突变体。分析了基因敲除株与野生型株生物学特性的差异。结果FoHog1基因编码357个氨基酸的蛋白质，并与镰刀菌不同的蛋白质有亲缘关系。与野生型菌株相比，FoHog1缺失突变株菌丝菌落松散，产孢量少，菌丝干重低，对温度、pH和渗透胁迫更敏感。与野生型菌株相比，FoHog1缺失突变体也具有降低的定殖能力。结论FoHog1基因参与了尖孢镰刀菌的菌丝生长、产孢、乙酸钠和氯化铵的降解、渗透胁迫反应及致病过程。古巴小种4.
OBJECTIVE This study was aimed to obtain a mitogen-activated protein kinase (MAPK) gene namely FoHog1 from Fusarium oxysporum f. sp. cubense and to verify its function. METHODS We amplified FoHog1 gene by PCR and RT-PCR methods and analyzed it through bioinformatics method. PEG-mediated protoplast transformation was used to create the deletion mutants of FoHog1 gene. We analyzed different biological characteristics between knock-out strain and wild-type strain. RESULTS FoHog1 gene encoding a putative protein of 357 amino acids and its genetic relationship with different Fusarium' s protein. Compared with the wild-type strain, FoHog1 deletion mutants have loose hyphae colony, less spores production, lower dry weight of hyphae and more sensitive to temperature, pH and osmotic stress. FoHog1 deletion mutants also have reduced colonization ability compared with the wild-type strain. CONCLUSION FoHog1 gene participated in mycelial growth, sporulation, catabolism of sodium acetate and ammonium chloride, osmotic stress response and pathogenic process with Fusarium oxysporum f. sp. cubense Race 4.