Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System.
Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System.
复制标题
DOI：
10.2147/ijn.s275258
复制
发表时间：
2020
影响因子：
8
通讯作者：
Bauab TM
中科院分区：
文献类型：
--
作者：
Gaspar de Toledo L;Dos Santos Ramos MA;Bento da Silva P;Rodero CF;de Sá Gomes V;Noronha da Silva A;Pavan FR;da Silva IC;Bombarda Oda F;Flumignan DL;Gonzaga Dos Santos A;Chorilli M;Gottardo de Almeida MT;Bauab TM
外阴阴道念珠菌病（VVC）是一种机会性真菌感染，由于令人不快的症状，治疗挑战和耐药菌株的出现，对女性的健康产生不利影响。天然产物和纳米技术的结合对于提高药用植物的抗真菌潜力非常重要。研究香茅挥发油微乳的体外和体内抗白色念珠菌活性。通过气相色谱-质谱法对EO进行化学分析。通过散射、zeta电位、偏振光显微镜、流变学测定、粘膜粘附和透射电子显微镜对ME和ME+EO进行表征。采用微量稀释法评价EO和ME+EO的体外抗真菌活性。在人类细胞系HaCat上分析了EO和ME+EO的毒性，并使用卤虫替代模型试验。在雌性小鼠（C57 BL/6）中进行了实验性体内VVC。发现EO的主要化合物为香茅醛、香叶醛、香叶醇、香茅醇和橙花醛。该制剂表现出适合阴道应用的尺寸、均匀性、负电荷、各向同性行为、高度有序的结构和假塑性行为。TEM显微照片显示球形结构内可能存在EO液滴。EO加入微胶囊后，其对念珠菌的抗真菌作用增强。白色念珠菌EO对卤虫无节幼体无毒性。体内VVC试验表明，ME的使用显著改善了EO的作用，因为在治疗的第三天，只有ME+EO促进了真菌阴道感染的根除。EO和ME+EO是控制C.白色念珠菌，一旦使用纳米技术显着提高了EO的抗真菌作用，特别是在VVC的体内模型。
Vulvovaginal candidiasis (VVC) is an opportunistic fungal infection that adversely affects a woman’s health, due to unpleasant symptoms, therapeutic challenges, and the emergence of resistant strains. The association of natural products and nanotechnology is important to improve the antifungal potential of medicinal plants. We aimed to evaluate the in vitro and in vivo anti-Candida albicans activity of unloaded (EO) and loaded (ME+EO) essential oil of Cymbopogon nardus in the microemulsion (ME). The chemical analysis of the EO was performed by gas chromatography-mass spectrometry. The ME and ME+EO were characterized by scattering, zeta potential, polarized light microscopy, rheological assays, mucoadhesiveness and transmission electronic microscopy. The in vitro antifungal activity of the EO and ME+EO were evaluated by microdilution technique. The toxicity of EO and ME+EO was analyzed on human cell line HaCat and using alternative model assay with Artemia salina. The experimental in vivo VVC was performed in female mice (C57BL/6). The main compounds of the EO were found to be citronellal, geranial, geraniol, citronellol, and neral. The formulations exhibited suitable size, homogeneity, negative charge, isotropic behavior, highly organized structure, and pseudoplastic behavior, for vaginal application. TEM photomicrographs showed possible EO droplets inside the spherical structures. The EO, when loaded into the ME, exhibited an improvement in its antifungal action against C. albicans. The EO was not toxic against brine shrimp nauplii. An in vivo VVC assay showed that the use of the ME significantly improved the action of the EO, since only the ME+EO promoted the eradication of the fungal vaginal infection on the third day of treatment. The EO and ME+EO are promising alternatives for the control of fungal infections caused by C. albicans, once the use of nanotechnology significantly improved the antifungal action of the EO, especially in an in vivo model of VVC.