African swine fever virus-specific cytotoxic T lymphocytes recognize the 32 kDa immediate early protein (vp32)
African swine fever virus-specific cytotoxic T lymphocytes recognize the 32 kDa immediate early protein (vp32)
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DOI：
10.1016/s0168-1702(97)01459-7
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发表时间：
1997-06-01
期刊：
影响因子：
5
通讯作者：
Revilla, Y
中科院分区：
文献类型：
--
作者：
Alonso, F;Dominguez, J;Revilla, Y
在SLA近交系小型猪感染非洲猪瘟病毒减毒株的模型中，研究了非洲猪瘟病毒特异性细胞毒性T淋巴细胞(CTL)的活性。以肺泡巨噬细胞为靶细胞进行CTL检测。这种特异性的溶解是由纯化的CD8(+)淋巴细胞介导的，而不是由CD4(+)细胞介导的，并且可以被抗SLA I类单抗所阻断。纯化的CD8(+)群体经ASF病毒刺激后产生高水平的干扰素-γ。为了确定CTL识别的病毒蛋白，将表达ASF病毒p32的重组痘苗病毒(VV)感染的靶细胞用CTL识别和裂解。该方法可用于VV重组筛选，以确定其他潜在的ASF病毒靶蛋白。(C)1997年爱思唯尔科学公司。
African swine fever (ASF) virus-specific cytotoxic T lymphocyte (CTL) activity has been studied in a model in which SLA inbred minipigs were experimentally infected with an attenuated isolate of the virus. The CTL assays were performed using alveolar macrophages as target cells. The specific lysis is mediated by purified CD8(+) lymphocytes but not by CD4(+) cells and can be blocked by incubation with anti-SLA class I monoclonal antibodies. The purified CD8(+) population produced high levels of interferon-gamma after ASF virus stimulation. In an attempt to define the Viral proteins recognized by CTL, target cells infected with a recombinant vaccinia virus (VV) expressing the ASF virus p32, an immediate early protein during ASF virus replication, were recognized and lysed by CTL. This assay may be useful for VV recombinant screening in order to identify other potential target ASF virus proteins. (C) 1997 Elsevier Science B.V.