Activated T lymphocytes migrate toward the cathode of DC electric fields in microfluidic devices
Activated T lymphocytes migrate toward the cathode of DC electric fields in microfluidic devices
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DOI：
10.1039/c0lc00371a
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Lin, Francis
中科院分区：
文献类型：
--
作者：
Li, Jing;Nandagopal, Saravanan;Lin, Francis
免疫细胞迁移是实现免疫监视和免疫应答的基本过程。了解免疫细胞迁移的机制不仅对细胞生物学具有重要意义，而且与细胞运输介导的生理过程和疾病如胚胎发生、伤口愈合、自身免疫性疾病和癌症具有高度相关性。除了众所周知的基于化学浓度梯度的引导机制（即趋化性）之外，最近的研究已经表明，淋巴细胞可以通过在体外和体内环境中向场的阴极迁移（即趋电性）来响应所施加的生理相关直流（DC）电场。在本研究中，我们采用了两个微流控装置，允许控制应用微流控通道内的电场定量研究淋巴细胞的趋电性在体外单细胞水平。第一个器件通过软光刻制造，第二个器件在玻璃中制造，具有集成的片上电极。使用这两种装置，我们首次表明，抗CD 3/CD 28抗体激活的人血T细胞迁移到所施加的直流电场的阴极。这一发现与以前对其他淋巴细胞亚群的趋电性研究一致，表明趋电性是免疫细胞迁移的一种新的指导机制。比较了活化T细胞在PDMS微流控器件中的趋电性和趋化性。
Immune cell migration is a fundamental process that enables immunosurveillance and immune responses. Understanding the mechanism of immune cell migration is not only of importance to the biology of cells, but also has high relevance to cell trafficking mediated physiological processes and diseases such as embryogenesis, wound healing, autoimmune diseases and cancers. In addition to the well-known chemical concentration gradient based guiding mechanism (i.e. chemotaxis), recent studies have shown that lymphocytes can respond to applied physiologically relevant direct current (DC) electric fields by migrating toward the cathode of the fields (i.e. electrotaxis) in both in vitro and in vivo settings. In the present study, we employed two microfluidic devices allowing controlled application of electric fields inside the microfluidic channel for quantitative studies of lymphocyte electrotaxis in vitro at the single cell level. The first device is fabricated by soft-lithography and the second device is made in glass with integrated on-chip electrodes. Using both devices, we for the first time showed that anti-CD3/CD28 antibodies activated human blood T cells migrate to the cathode of the applied DC electric field. This finding is consistent with previous electrotaxis studies on other lymphocyte subsets suggesting electrotaxis is a novel guiding mechanism for immune cell migration. Furthermore, the characteristics of electrotaxis and chemotaxis of activated T cells in PDMS microfluidic devices are compared.