Polymorphism of genes encoding toll-like receptors and inflammatory cytokines in periodontal disease in the Japanese population.
Polymorphism of genes encoding toll-like receptors and inflammatory cytokines in periodontal disease in the Japanese population.
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日本人群牙周病中 Toll 样受体和炎症细胞因子编码基因的多态性。
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Pao‐Li Wang
中科院分区：
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作者：
Y. Imamura;Yoshihisa Fujigaki;Y. Oomori;T. Kuno;N. Ota;Pao‐Li Wang
牙周炎是一种广泛存在的主要牙科疾病，是一种与生活方式相关的多因素疾病，已被分析为基因多态。我们研究了促炎症细胞因子基因IL-1A(-889)和IL-1B(+3953)的多态在日本人群中与牙周炎的关系。我们还检测了牙周病细菌识别的受体TLR2(Arg677Trp)和TLR4(Asp299Gly)的多态性是否也与牙周炎有关。研究对象为92名日本人，其中43人患有牙周炎，49人为健康对照。我们从舌粘膜细胞中提取基因组DNA，并用聚合酶链式反应-限制性片段长度多态性方法检测它们的单核苷酸多态性。用Fisher‘s精确检验对基因多态的发生率进行统计学分析，并计算基因多态的敏感性和特异性。目的是确定这些基因多态性是否可以有效地用于牙周炎的诊断。然而，我们没有发现IL-1A(p=0.082)、IL-1B(p=0.180)、TLR2(p=1.000)或TLR4(p=1.000)的基因多态以及任何一个基因的总基因多态(p=0.752)与牙周炎相关。所有基因突变的敏感性(14.0%)和特异性(83.7%)均较低。因此，我们建议不要使用这些基因的多态分析来检测日本人群中的牙周炎。
Periodontitis, which is a widespread and major dental disease, is a multifactorial, lifestyle-related disease and has been analyzed for gene polymorphism. We examined the frequency of the polymorphisms of the pro-inflammatory cytokine genes IL-1 A (-889) and IL-1 B (+3953) in relation to periodontitis in the Japanese population. We also examined whether polymorphism of TLR2 (Arg677Trp) and TLR4 (Asp299Gly), which are receptors recognized by periodontopathic bacteria, may also be associated with periodontitis. The subjects were 92 Japanese individuals, among whom 43 had periodontitis and 49 were healthy controls. We isolated genomic DNA from lingual mucosal cells and tested them for single nucleotide polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. The incidence of polymorphisms was analyzed statistically by Fisher's exact test, and the sensitivity and specificity of the gene polymorphisms were calculated. The purpose was to determine whether such polymorphisms might be effectively used in the diagnosis of periodontitis. However, we found no evidence that the gene polymorphism of IL-1A (p = 0.082), IL-1 B (p = 0.180), TLR2 (p = 1.000) or TLR4 (p = 1.000) and overall gene polymorphism in any of the genes (p = 0.752) correlate with periodontitis. The sensitivity (14.0%) and specificity (83.7%) of the mutations found in all of the genes were low. Therefore, we advise against using the analyses of polymorphism of these genes to detect periodontitis in the Japanese population.