Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)
Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)
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DOI：
10.1016/s0165-2427(98)00156-1
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发表时间：
1998-10-23
影响因子：
1.8
通讯作者：
Harbour, DA
中科院分区：
文献类型：
--
作者：
Gunn-Moore, DA;Caney, SMA;Harbour, DA
两种重组FIPV刺突蛋白在8周龄的小猫身上进行了免疫原性评估，然后用FIPV79-1146鼻腔攻击小猫。体液反应采用酶联免疫吸附试验和血清中和试验进行评估。采用逆转录半定量聚合酶链式反应(RT-SQPCR)检测PBMC细胞因子mRNA水平的变化，检测IL-2、IL-4、IL-6、IL-10、IL-12和干扰素-γ水平。所有的小猫都出现了典型的FIP临床症状，这些症状在尸检中得到了证实。与对照组相比，重组蛋白在攻击前几乎没有或几乎没有诱导特异性抗体反应，并且未能改变疾病的进程。病毒攻击后1周，受刺激的PBMCs IL-6和干扰素-γmRNA表达略有增加，与一过性发热有关。在此之后，IL-6mRNA的表达没有变化，但随着FIP的发展，IL-2、IL-4、IL-10、IL-12和干扰素-γ的mRNA水平明显下降。(C)1998 Elsevier Science B.V.保留所有权利。
Two recombinant FIPV spike proteins were assessed for their immunogenic properties in 8-week-old kittens, which were then challenged intranasally with FIPV 79-1146. Humoral responses were assessed by ELISA and serum neutralisation test. Changes in PBMC cytokine mRNA levels were detected by a reverse transcription, semiquantitative polymerase chain reaction assay (RT-sqPCR), assessing IL-2, IL-4, IL-6, IL-10, IL-12 and IFN gamma. All of the kittens developed clinical signs typical of FIP, which were confirmed on gross post mortem examination. The recombinant proteins induced little or no specific antibody response prior to challenge, and failed to alter the course of disease compared to controls. One week after virus challenge, the stimulated PBMCs showed small increases in the expression of IL-6 and IFN gamma, mRNA, which correlated with a transient pyrexia. After this time expression of IL-6 mRNA remained unaltered but, as FIP developed, mRNA levels of IL-2, IL-4, IL-10, IL-12 and IFN gamma became markedly depressed. (C) 1998 Elsevier Science B.V. All rights reserved.