Cloning, identification, and functional analysis of bone marrow stromal cell antigen-2 from sika deer (Cervus nippon)
Cloning, identification, and functional analysis of bone marrow stromal cell antigen-2 from sika deer (Cervus nippon)
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梅花鹿骨髓基质细胞抗原2的克隆、鉴定及功能分析
DOI：
10.1016/j.gene.2018.03.101
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Zhao Yu
中科院分区：
文献类型：
--
作者：
Wang Jiawen;Bian Shuai;Liu Meichun;Zhang Xin;Wang Siming;Bai Xueyuan;Zhao Daqing;Zhao Yu
BST-2（tetherin/CD 317/HM 1.24）已被鉴定为细胞抗病毒因子，其抑制多种包膜病毒从感染细胞中释放。BST-2的直系同源物已在包括人、猴、牛、羊、猪和小鼠的几个物种中鉴定。本研究克隆了西卡BST-2同源基因，并对其蛋白质进行了分析。cnBST-2与人BST-2同源物的同源性为37.8%，与绵羊BST-2同源物的同源性为74.2%。cnBST-2的胞外结构域具有两个推定的N-连接的糖基化位点和三个潜在的二聚化位点。cnBST-2显示在细胞表面上表达，像人BST-2一样。cnBST-2的外源性表达导致293 T细胞中HIV-1颗粒释放的有效抑制;然而，这种活性抵抗HIV-1 Vpu的拮抗作用。此外，与人BST-2相反，cnBST-2不能激活核因子-κB。本研究首次报道了从日本沼虾中分离得到BST-2的分离纯化和鉴定。
BST-2(tetherin/CD317/HM1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. Orthologs of BST-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. In this study, we cloned the gene and characterized the protein of the BST-2 homolog from sika deer (Cervus nippon). cnBST-2 shares 37.8% and 74.2% identity with the BST-2 homologs from Homo sapiens andOvisaries, respectively. The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites. cnBST-2 was shown to be expressed on the cell surface, like human BST-2. Exogenous expression of cnBST-2 resulted in potent inhibition of HIV-1 particle release in 293T cells; however, this activity resisted antagonism by HIV-1 Vpu. Moreover, cnBST-2 was not able to activate nuclear factor-κB, in contrast to human BST-2. This study is the first report of the isolation and characterization of BST-2 fromC.nippon.