Role of serum CXCL9 and CXCL13 in predicting infection after kidney transplant: A STROBE study.
Role of serum CXCL9 and CXCL13 in predicting infection after kidney transplant: A STROBE study.
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DOI：
10.1097/md.0000000000024762
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发表时间：
2021-03-19
期刊：
影响因子：
1.6
通讯作者：
Shi YY
中科院分区：
文献类型：
--
作者：
Yan L;Li YM;Li Y;Bai YJ;Wan ZL;Fan JW;Luo LM;Wang LL;Shi YY
趋化因子主要参与炎症和免疫反应。干扰素-γ诱导的趋化因子C-X-C基序趋化因子9和10（CXCL9和CXCL10）与Th1细胞和单核细胞密切相关，并且它们的表达水平在同种肾移植排斥和各种感染性疾病的早期发作期间迅速增加。 CXCL13 是最有效的 B 细胞和 T 滤泡辅助细胞化学引诱剂之一。 CXCL13 在感染时的表达表明在多种感染性疾病中具有重要的趋化活性。 C-C 基序趋化因子配体 2 (CCL2) 可以在炎症反应期间吸引单核细胞和巨噬细胞。然而，目前还没有关于这些趋化因子在肾移植受者移植后感染中的作用的研究。在本研究中，使用 Bio-Plex 悬浮芯片系统在移植前和移植后 30 天对 CXCL9、CXCL10、CXCL13 和 CCL2 进行分析。肾移植后30天CXCL9和CXCL13的血清水平与移植后1年内的感染相关（分别为P = .021和P = .002）。术前血清CXCL9、CXCL13以及术前、术后血清CCL2、CXCL10水平与移植后1年内感染无关（P > .05）。术后第 30 天 CXCL9 和术后第 30 天 CXCL13 的组合提供了最佳结果，曲线下面积为 0.721（95% 置信区间，0.591–0.852），在最佳截止值 52.72 pg/mL 时，敏感性为 71.4%，特异性为 68.5%。 CXCL9和CXCL13作为重要的趋化因子，可用于预测肾移植术后感染的发生。
Chemokines are majorly involved in inflammatory and immune responses. The interferon-γ-inducible chemokines C-X-C motif chemokines 9 and 10 (CXCL9 and CXCL10) are considerably associated with Th1 cells and monocytes, and their expression levels rapidly increase during the early episodes of renal allograft rejection and various infectious diseases. CXCL13 is one of the most potent B-cell and T follicular helper-cell chemoattractants. The expression of CXCL13 in the presence of infection indicates an important chemotactic activity in multiple infectious diseases. C-C motif chemokine ligand 2 (CCL2) can attract monocytes and macrophages during inflammatory responses. However, there are no studies on the role of these chemokines in posttransplant infection in kidney transplant recipients. In this study, CXCL9, CXCL10, CXCL13, and CCL2 were analyzed using the Bio-Plex suspension array system before transplant and 30 days after transplant. The serum levels of CXCL9 and CXCL13 30 days after kidney transplant were associated with infection within 1 year after transplant (P = .021 and P = .002, respectively). The serum levels of CXCL9 and CXCL13 before surgery and those of CCL2 and CXCL10 before and after surgery were not associated with infection within 1 year after transplant (P > .05). The combination of postoperative day (POD) 30 CXCL9 and postoperative day 30 CXCL13 provided the best results with an area under the curve of 0.721 (95% confidence interval, 0.591–0.852), with a sensitivity of 71.4% and specificity of 68.5% at the optimal cutoff value of 52.72 pg/mL. As important chemokines, CXCL9 and CXCL13 could be used to predict the occurrence of infection after kidney transplant.