Inhibition of bacterial RNA polymerase by the cyanobacterial metabolites 12-epi-hapalindole E isonitrile and calothrixin A
Inhibition of bacterial RNA polymerase by the cyanobacterial metabolites 12-epi-hapalindole E isonitrile and calothrixin A
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DOI：
10.1016/s0378-1097(01)00059-3
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发表时间：
2001-03-15
影响因子：
2.1
通讯作者：
Smith, GD
中科院分区：
文献类型：
--
作者：
Doan, NT;Stewart, PR;Smith, GD
来自蓝细菌 Fischerella 物种的生物碱 12-epi-hapalindole E 异腈和来自 Calothrix 的吲哚菲啶 calothrixin A，对 ATP 具有竞争性抑制大肠杆菌 RNA 聚合酶，而对 UTP 则具有非竞争性抑制作用。抑制取决于抑制剂的添加顺序。以 ATP 作为可变底物时，K-I 值分别为 1.3 +/- 0.2 mM 和 0.23 +/- 0.11 mM。根据与全细胞对这些抑制剂的敏感性的比较：得出的结论是，除了 RNA 聚合酶之外，其他靶标也可能参与其作用。 (C) 2001 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
The alkaloid 12-epi-hapalindole E isonitrile, from a cyanobacterial Fischerella species, and the indolophenanthridine calothrixin A, from Calothrix, inhibited Escherichia coli RNA polymerase competitively with respect to ATP, and non-competitively with respect to UTP. The inhibition was dependent on the order of addition of the inhibitors. The K-I values, with ATP as the variable substrate, were 1.3 +/- 0.2 mM and 0.23 +/- 0.11 mM, respectively. Based on comparisons with the sensitivity of whole cells to these inhibitors: it is concluded that other targets in addition to RNA polymerase may also be implicated in their action. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.