Topoisomerase II cleavage of herpes simplex virus type 1 DNA in vivo is replication dependent.
Topoisomerase II cleavage of herpes simplex virus type 1 DNA in vivo is replication dependent.
复制标题
拓扑异构酶 II 在体内对 1 型单纯疱疹病毒 DNA 的切割具有复制依赖性。
DOI：
10.1128/jvi.64.9.4059-4066.1990
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发表时间：
1990
影响因子：
5.4
通讯作者：
Muller,MT
中科院分区：
文献类型：
--
作者：
Ebert,SN;Shtrom,SS;Muller,MT
单纯疱疹病毒1型的基因组中含有大量的真核DNA Ⅱ型拓扑异构酶的识别位点。拓扑异构酶II位点通过先前描述的共有序列鉴定（J.R. Spitzner和M.T. Muller，Nucleic Acids Res. 16：5553-5556，1988），然后通过对由纯化的拓扑异构酶II引入的DNA裂解进行测序来确认。在体内，宿主拓扑异构酶II也在病毒基因组中由共有序列预测的位点处引入双链DNA断裂。宿主拓扑异构酶II作用于所有立即早期基因以及其他时间类的基因;然而，直到感染后4至5小时才检测到裂解，并且在感染后10小时最强烈。拓扑异构酶II裂解时，没有检测到病毒DNA复制与膦酰基乙酸阻止。这些数据表明，虽然子代病毒基因组上的主机拓扑异构酶II的作用，这种酶要么不作用于父母的病毒基因组DNA复制前或作用于他们的效率如此之低，裂解超出了我们的检测极限。研究结果表明，宿主拓扑异构酶II参与了感染周期后期病毒复制的各个方面。
The genome of herpes simplex virus type 1 contains a large number of recognition sites for eucaryotic DNA type II topoisomerase. Topoisomerase II sites were identified by means of the consensus sequence described previously (J.R. Spitzner and M.T. Muller, Nucleic Acids Res. 16:5553-5556, 1988) and then confirmed by sequencing DNA cleavages introduced by purified topoisomerase II. In vivo, host topoisomerase II also introduced double-stranded DNA breaks in the viral genome at sites predicted by the consensus sequence. Host topoisomerase II acted on all immediate-early genes as well as on genes from other temporal classes; however, cleavages were not detected until 4 to 5 h postinfection and were most intense at 10 h postinfection. Topoisomerase II cleavages were not detected when viral DNA replication was prevented with phosphonoacetic acid. These data indicate that, although progeny viral genomes are acted upon by host topoisomerase II, this enzyme either does not act on parental viral genomes before DNA replication or acts on them with such low efficiency that cleavages are beyond our limit of detection. The findings suggest that host topoisomerase II is involved in aspects of viral replication at late times in the infectious cycle.