Chloroplast DNA microsatellite analysis supports a polyphyletic origin for barley
Chloroplast DNA microsatellite analysis supports a polyphyletic origin for barley
复制标题
DOI：
10.1007/s00122-004-1878-3
复制
发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Powell, W
中科院分区：
文献类型：
--
作者：
Molina-Cano, JL;Russell, JR;Powell, W
利用5个大麦叶绿体DNA微卫星（cpSSR）标记，研究了34个普通大麦（Hordeum vulgare ssp.）来自摩洛哥、埃塞俄比亚、塞浦路斯、克里特岛、利比亚、伊拉克、伊朗、土耳其、阿富汗和以色列的割手密（HS加入），122 H. vulgare ssp.来自西班牙、玻利维亚（古老的西班牙引进品种）、摩洛哥、利比亚和埃塞俄比亚的vulgare地方品种（HV地方品种）和20种现代欧洲春大麦（HV栽培品种）。所有位点的研究材料中的多态性，每个位点的等位基因数从2到3不等。15个多位点单倍型进行了观察，11个在HS加入和HV地方品种和品种7。在HV品系中发现的7个单倍型中，3个与HS品系共有，4个是独特的。聚类分析显示两个主要群体，一个由来自埃塞俄比亚的HS加入和来自西班牙、玻利维亚（旧西班牙）、摩洛哥和埃塞俄比亚的HV地方品种组成，而另一个较大的群体包含所有其他加入的研究。基于这些分组和存在的单倍型HV地方品种和栽培品种，但不是在HS野生大麦，提出了一个多系起源大麦，在埃塞俄比亚和西地中海的起源中心。
Five barley chloroplast DNA microsatellites (cpSSRs) were used to study genetic relationships among a set of 186 barley accessions - 34 Hordeum vulgare ssp. spontaneum (HS accessions) from Morocco, Ethiopia, Cyprus, Crete, Libya, Iraq, Iran, Turkey, Afghanistan and Israel, 122 H. vulgare ssp. vulgare landraces (HV landraces) from Spain, Bolivia (old Spanish introductions), Morocco, Libya and Ethiopia and 20 modern European spring barleys (HV cultivars). All loci were polymorphic in the material studied, with the number of alleles per locus ranging from two to three. Fifteen multi-locus haplotypes were observed, 11 in HS accessions and seven in HV landraces and cultivars. Of the seven haplotypes found in the HV lines, three were shared with the HS accessions, and four were unique. Cluster analysis revealed two main groups, one consisting of HS accessions from Ethiopia and the HV landraces from Spain, Bolivia (old Spanish), Morocco and Ethiopia, whereas the other larger group contained all of the other accessions studied. Based on these grouping and the existence of haplotypes found in the HV landraces and cultivars but not in the HS wild barley, a polyphyletic origin is proposed for barley, with further centres of origin in Ethiopia and the Western Mediterranean.