Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine
Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine
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DOI：
10.1016/j.biomaterials.2006.06.033
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发表时间：
2006-12-01
期刊：
影响因子：
14
通讯作者：
Elisseeff, Jennifer
中科院分区：
文献类型：
--
作者：
Hwang, Nathaniel S.;Varghese, Shyni;Elisseeff, Jennifer
胚胎干（ES）细胞的分化通常发生在形成三维细胞聚集体（称为胚状体（EB））之后。我们之前已经报道了水凝胶为EB提供了体外软骨形成分化和三维组织形成的支持环境[Hwang NS等人，The Effects of three dimensional culture and growth factors on the chondrogenic differentiation of murine ES cells.干细胞2006;24：284-91]。在这项研究中，我们报告软骨分化的小鼠ES细胞封装在光聚合的聚（乙二醇）为基础的（PEG）水凝胶中存在的葡萄糖胺（GlcN），氨基单糖中发现的几丁质，糖蛋白和糖胺聚糖，如透明质酸，硫酸软骨素和硫酸肝素。我们研究了在含有0-，2-和10-mm GlcN的标准成软骨分化培养基中封装的EB的生长和分化。形态分析和基因和蛋白质表达的检查表明，治疗水凝胶培养物与2毫米GlcN 21天显着增加EB大小，聚集蛋白聚糖mRNA的水平，和组织特异性细胞外基质积累。GlcN可以诱导细胞行为的多个方面，并且最佳GlcN浓度可以有益于指导ES细胞的分化和组织形成。(c)2006爱思唯尔有限公司保留所有权利。
Differentiation of embryonic stem (ES) cells generally occurs after formation of three-dimensional cell aggregates, known as embryoid bodies (EBs). We have previously reported that hydrogels provide EBs a supportive environment for in vitro chondrogenic differentiation and three dimensional tissue formation [Hwang NS, et al. The Effects of three dimensional culture and growth factors on the chondrogenic differentiation of murine ES cells. Stem Cells 2006;24:284-91]. In this study, we report chondrogenic differentiation of murine ES cells encapsulated in photopolymerizing poly(ethylene-glycol)-based (PEG) hydrogels in the presence of glucosamine (GlcN), an amino monosaccharide found in chitin, glycoproteins and glycosaminoglycans such as hyaluronic acid, chondroitin sulfate and heparin sulfate. We examined the growth and differentiation of encapsulated EBs in standard chondrogenic differentiation medium containing 0-, 2-, and 10-mm GlcN. Morphometric analysis and examination of gene and protein expression indicated that treatment of hydrogel cultures with 2-mm GlcN for 21 days significantly increased EB size, levels of aggrecan mRNA, and tissue-specific extracellular matrix accumulation. GlcN can induce multiple aspects of cell behavior and optimal GlcN concentrations can be beneficial for directing the differentiation and tissue formation of ES cells. (c) 2006 Elsevier Ltd. All rights reserved.