Plasmid DNA profiles as epidemiological markers for clinical and environmental isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum.
Plasmid DNA profiles as epidemiological markers for clinical and environmental isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum.
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质粒 DNA 谱作为鸟分枝杆菌、胞内分枝杆菌和瘰疬分枝杆菌临床和环境分离株的流行病学标记。
DOI：
10.1093/infdis/153.2.325
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Falkinham3rd,JO
中科院分区：
文献类型：
--
作者：
Meissner,PS;Falkinham3rd,JO
分离质粒DNA，并比较各种鸟分枝杆菌、胞内分枝杆菌和结核分枝杆菌（迈斯）的临床和环境分离株的图谱，以确定质粒DNA含量是否可用作这些环境病原体的流行病学标记。由于质粒在临床分离株中很常见，并且在培养和暴露于NaOH期间稳定，因此质粒DNA分析似乎是一种合适的流行病学工具。基于仅临床（56%）和气溶胶（75%）分离株中质粒的高频率以及土壤（5%）、灰尘（7%）、沉积物（<6%）和水（25%）分离株中质粒的低频率，数据表明，美国东南部沃茨上空产生的载有MAIS的气溶胶可能是人类感染的来源。
Plasmid DNA was isolated, and profiles of a variety of clinical and environmental isolates ofMycobacterium avium, Mycobacterium intracellulare, andMycobacterium scrofulaceum(MAIS) were compared to determine whether plasmid DNA content would be useful as an epidemiological marker for these environmental pathogens. Since plasmids are common in clinical isolates and are stable during culture and exposure to NaOH, plasmid DNA analysis appears to be a suitable epidemiological tool. Based on the high frequency of plasmids in only clinical (56%) and aerosol (75%) isolates and low frequencies in soil (5%), dust (7%), sediment (<6%), and water (25%) isolates, the data suggest that MAIS-laden aerosols generated over waters of the southeastern United States are a likely source of human infection.