PREFERENTIAL INHIBITION OF PRIMARY GRANULE RELEASE FROM BOVINE NEUTROPHILS BY A BRUCELLA-ABORTUS EXTRACT
PREFERENTIAL INHIBITION OF PRIMARY GRANULE RELEASE FROM BOVINE NEUTROPHILS BY A BRUCELLA-ABORTUS EXTRACT
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DOI：
10.1128/iai.52.1.285-292.1986
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发表时间：
1986-04-01
影响因子：
3.1
通讯作者：
ROTH, JA
中科院分区：
文献类型：
--
作者：
BERTRAM, TA;CANNING, PC;ROTH, JA
一种低分子量的流产布鲁氏菌提取物（一种核苷酸样物质）抑制酵素诱导的中性粒细胞脱粒和三氯乙酸沉淀蛋白碘化（中性粒细胞释放髓过氧化物酶和H2O2的一种测量方法）。对中性粒细胞功能的抑制作用与布鲁氏菌提取物的浓度直接相关。提取物优先抑制初级颗粒（亲氮或过氧化物酶阳性）的脱粒，对次级颗粒（特异性或过氧化物酶阴性）的释放有有限的抑制，但不抑制调理酶的摄入。蛋白质碘化的抑制作用与一次颗粒释放密切相关，但与二次颗粒释放的抑制无关。这些结果表明，牛肉芽孢杆菌具有一种能够通过剂量依赖的优先抑制牛中性粒细胞原代颗粒释放来抑制髓过氧化物酶释放的成分。
A low-molecular-weight Brucella abortus extract (a nucleotidelike material) inhibited zymosan-elicited neutrophil degranulation and trichloroacetic acid-precipitable protein iodination (a measure of myeloperoxidase and H2O2 release from neutrophilic leukocytes). Inhibition of neutrophil function was directly related to the concentration of the Brucella extract. The extract preferentially inhibited degranulation of primary (azurophilic or peroxidase positive) granules and had limited inhibition of secondary (specific or peroxidase negative) granule release but did not inhibit opsonized zymosan ingestion. Inhibition of protein iodination closely paralleled that of primary granule release but was unrelated to inhibition of secondary granule release. These results suggest that B. aborutus has a component which is capable of inhibiting release of myeloperoxidase by dose-dependent preferential inhibition of primary granule release from bovine neutrophilic leukocytes.