Organoids as a tool for understanding immune-mediated intestinal regeneration and development
Organoids as a tool for understanding immune-mediated intestinal regeneration and development
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DOI：
10.1242/dev.199904
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发表时间：
2022-04-01
期刊：
影响因子：
4.6
通讯作者：
Neves, Joana F.
中科院分区：
文献类型：
--
作者：
Jowett, Geraldine M.;Coales, Isabelle;Neves, Joana F.
免疫细胞的传统观点是它们在体内的作用是对抗感染;然而，越来越清楚的是，它们也执行与炎症和病原体清除无关的任务。这些功能是在组织深处执行的，这些组织通常很难接近，并受到环境变化的影响，特别是在人类中。在这里，我们讨论了培养皿中的多细胞3D系统-类器官-如何从原理证明方法过渡到及时，强大和可靠的工具。虽然我们主要关注与淋巴细胞共培养的肠道类器官的最新研究结果，但我们认为类器官共培养系统将支持未来的努力，以解开整个发育，稳态，再生和疾病中大量不同细胞类型之间的相互作用。
The traditional view of immune cells is that their role within the body is to combat infections; however, it is becoming increasingly clear that they also perform tasks that are not classically associated with inflammation and pathogen clearance. These functions are executed deep within tissues, which are often poorly accessible and subject to environmental variability, especially in humans. Here, we discuss how multicellular 3D systems in a dish - organoids - are transitioning from a proof-of-principle approach to a timely, robust and reliable tool. Although we primarily focus on recent findings enabled by intestinal organoids co-cultured with lymphocytes, we posit that organoid coculture systems will support future efforts to disentangle the interactions between a plethora of different cell types throughout development, homeostasis, regeneration and disease.