Escherichia coli 4.5S RNA gene function can be complemented by heterologous bacterial RNA genes.
Escherichia coli 4.5S RNA gene function can be complemented by heterologous bacterial RNA genes.
复制标题
大肠杆菌4.5S RNA基因的功能可以通过异源细菌RNA基因来补充。
DOI：
10.1128/jb.172.3.1284-1288.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Fournier,MJ
中科院分区：
文献类型：
--
作者：
Struck,JC;Lempicki,RA;Toschka,HY;Erdmann,VA;Fournier,MJ
大肠杆菌的必需4.5S RNA基因可以由来自其他三种真细菌的4.5S RNA样基因互补，包括革兰氏阳性和革兰氏阴性生物。其中两个基因编码的RNA大小与E.第三种，来自枯草芽孢杆菌，指定了一种两倍以上的RNA。外源基因在大肠杆菌中得到了高效表达。大肠杆菌，和产品RNA类似的同源细胞产生的。我们的结论是，异源RNA可以取代E。coli 4.5S RNA的基本功能在进化上是保守的。一个共识结构的功能相关的4.5S RNA同系物。
The essential 4.5S RNA gene of Escherichia coli can be complemented by 4.5S RNA-like genes from three other eubacteria, including both gram-positive and gram-negative organisms. Two of the genes encode RNAs similar in size to the E. coli species; the third, from Bacillus subtilis, specifies an RNA more than twice as large. The heterologous genes are expressed efficiently in E. coli, and the product RNAs resemble those produced by cognate cells. We conclude that the heterologous RNAs can replace E. coli 4.5S RNA and that the essential function of 4.5S RNA is evolutionarily conserved. A consensus structure is presented for the functionally related 4.5S RNA homologs.