Astrocytic exocytosis vesicles and glutamate: A high-resolution immunofluorescence study
Astrocytic exocytosis vesicles and glutamate: A high-resolution immunofluorescence study
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DOI：
10.1002/glia.20094
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发表时间：
2005-01-01
期刊：
影响因子：
6.2
通讯作者：
Derouiche, A
中科院分区：
文献类型：
--
作者：
Anlauf, E;Derouiche, A
生理证据表明，培养的星形胶质细胞可以通过Ca2+依赖机制释放谷氨酸。此外，海马片星形胶质细胞释放的谷氨酸干扰突触神经传递。由于这些观察结果表明星形胶质细胞释放谷氨酸囊泡，因此研究了含有谷氨酸的胞吐囊泡的存在。我们将免疫荧光技术与高性能反褶积显微镜相结合，其分辨率为
Physiological evidence has demonstrated that cultured astrocytes can release glutamate via Ca2+-dependent mechanisms. Also, glutamate released from astrocytes in the hippocampal slice interferes with synaptic neurotransmission. Since these observations suggest vesicular glutamate release from astrocytes, the presence of glutamate-containing exocytosis vesicles was investigated. We applied immunofluorescence techniques combined with high-performance deconvolution microscopy, which yields a resolution of