Role of Astroglial Connexin30 in Hippocampal Gap Junction Coupling
Role of Astroglial Connexin30 in Hippocampal Gap Junction Coupling
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DOI：
10.1002/glia.21120
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发表时间：
2011-03-01
期刊：
影响因子：
6.2
通讯作者：
Theis, Martin
中科院分区：
文献类型：
--
作者：
Gosejacob, Dominic;Dublin, Pavel;Theis, Martin
连接蛋白 30 (Cx30) 对正常海马星形细胞间隙连接耦合的影响仍存在争议。报告基因分析表明 Cx30 在小鼠海马体中表达较弱。相比之下，星形胶质细胞中缺乏连接蛋白43 (Cx43) 的小鼠的耦合仅减少 50%。同时缺乏 Cx30 和 Cx43 的小鼠海马星形胶质细胞的完全解偶联表明 Cx30 参与海马中的星形细胞间隙连接偶联。通过比较报告基因测定、免疫检测和基于 cre/loxP 的报告方法，我们证明 Cx30 比之前想象的更丰富。 Cx30 在星形细胞间耦合中的具体作用从未被研究过。通过对 Cx30 缺陷小鼠的急性切片进行示踪偶联分析，我们发现 Cx30 对小鼠海马体星形细胞间隙连接通讯做出了重大贡献。 (C)2010 Wiley-Liss, Inc.
The impact of connexin30 (Cx30) on interastrocytic gap junction coupling in the normal hippocampus is matter of debate; reporter gene analyses indicated a weak expression of Cx30 in the mouse hippocampus. In contrast, mice lacking connexin43 (Cx43) in astrocytes exhibited only 50% reduction in coupling. Complete uncoupling of hippocampal astrocytes in mice lacking both Cx30 and Cx43 suggested that Cx30 participates in interastrocytic gap junction coupling in the hippocampus. With comparative reporter gene assays, immunodetection, and cre/loxP-based reporter approaches we demonstrate that Cx30 is more abundant than previously thought. The specific role of Cx30 in interastrocytic coupling has never been investigated. Employing tracer coupling analyses in acute slices of Cx30 deficient mice here we show that Cx30 makes a substantial contribution to interastrocytic gap junctional communication in the mouse hippocampus. (C)2010 Wiley-Liss, Inc.