Electrophysiological Measurement of Rat Atrial Epicardium Using a Novel Stereotaxic Apparatus
Electrophysiological Measurement of Rat Atrial Epicardium Using a Novel Stereotaxic Apparatus
复制标题
使用新型立体定向装置对大鼠心房外膜进行电生理测量
DOI：
10.1536/ihj.18-215
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发表时间：
2019-03-01
影响因子：
1.5
通讯作者：
Zheng, Qiangsun
中科院分区：
文献类型：
--
作者：
Fan, Boyuan;Wang, Hongtao;Zheng, Qiangsun
灵活的、体内可重复的电生理标测技术在大鼠模型中不可用。一种新型心脏立体定向电生理心外膜标测系统（CREAMS）允许进行各种测量，包括：（1）记录多个部位的单极电描记图;（2）标测部位的定位和精度测试（两个“中心”之间的距离= 297 +/- 54 μ m，n = 15）;（3）在具有高频刺激（HFS）诱导的高右心房（HRA）部位的心房颤动（AF）的体内Sprague-Dawley大鼠模型中的电生理学评估。结果发现，HRA HFS后右心房有效不应期离散度（P < 0.05）和易损窗（P < 0.01）显著增加（P < 0.05）。CREAMS具有通过立体定位和灵活的操作操作在大鼠AF模型中进行方便的电生理学评估的潜力。
Flexible, in vivo maneuverable electrophysiology mapping techniques are not available in rat models. A novel cardiac stereotactic electrophysiology epicardial mapping system (CREAMS) allows for various measurements, including: (1) recording unipolar electrograms at multiple sites; (2) positioning of mapped sites and precision testing (Distance between the two "centers" = 297 +/- 54 mu m, n = 15); (3) evaluation of electrophysiology in an in vivo Sprague-Dawley rat model with high-frequency stimulation (HFS)-induced Atrial fibrillation (AF) at high right atrium (HRA) sites. We found that of the right atrium dispersion of effective refractory period (P < 0.05) and the window of vulnerability (P < 0.01) were significantly increased (P < 0.05) after HRA HFS. CREAMS has the potential for convenient electrophysiology assessment in a rat AF model through stereo-positioning, and flexible operating manipulation.