Effect of Bangle (Zingiber purpureum) extract and low-intensity exercise on mTOR phosphorylation and autophagy flux in skeletal muscles of rats on a high-fat diet.
Effect of Bangle (Zingiber purpureum) extract and low-intensity exercise on mTOR phosphorylation and autophagy flux in skeletal muscles of rats on a high-fat diet.
复制标题
Bangle（紫姜）提取物和低强度运动对高脂饮食大鼠骨骼肌 mTOR 磷酸化和自噬流的影响。
DOI：
10.1016/j.jff.2018.06.010
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发表时间：
2018
影响因子：
5.6
通讯作者：
Mukai Y
中科院分区：
文献类型：
--
作者：
Sato S;Kataoka S;Sato M;Takahashi A;Norikura T;Mukai Y
高脂肪饮食（HFD）与胰岛素敏感性受损有关。我们研究了手镯 (Zingiber purpureum) 提取物 (Ba) 结合运动训练 (Ex) 对 Akt 和哺乳动物雷帕霉素靶蛋白 (mTOR) 磷酸化以及 HFD 喂养大鼠腓肠肌 (GM) 自噬流的影响。将饮食中 45% 的能量来自脂肪的大鼠分为四组：未处理组 (HFD)、1.5% Ba 处理组 (HFD+Ba)、1.5% Ba 加 Ex 处理组 (HFD+Ba+Ex) 和 Ex 处理组 (HFD+Ex)。对照组接受正常饮食。 HFD+Ba+Ex 中胰岛素抵抗指数和非酯化脂肪酸水平的稳态模型评估降低。与 HFD 相比，HFD+Ba+Ex 显示磷酸化 Akt 和 mTOR 水平降低，自噬流标记物 LC3B-II 和 Beclin 1 水平升高。总之，Ba 和 Ex 的组合可调节 GM 中的 Akt/mTOR 磷酸化和自噬通量，并可能减弱 HFD 诱导的胰岛素抵抗。
High-fat diet (HFD) is associated with impairment of insulin sensitivity. We investigated the effects of Bangle (Zingiber purpureum) extract (Ba) with exercise training (Ex) on phosphorylation of Akt and mammalian target of rapamycin (mTOR), and autophagy flux in the gastrocnemius (GM) of HFD-fed rats. The rats, on a diet providing 45% of energy from fat, were divided into four groups: untreated (HFD), 1.5% Ba-treated (HFD + Ba), 1.5% Ba with Ex-treated (HFD + Ba + Ex), and Ex-treated (HFD + Ex) groups. The controls were fed a normal diet. The homeostatic model assessment of insulin resistance index and non-esterified fatty acid levels were decreased in the HFD + Ba + Ex. The HFD + Ba + Ex showed decreased phosphorylated Akt and mTOR levels, and increased levels of autophagy flux markers, LC3B-II and Beclin 1, compared to the HFD. In conclusion, the combination of Ba and Ex modulates Akt/mTOR phosphorylation and the autophagy flux in the GM and may attenuate insulin resistance induced by a HFD.