Amphiphilic agarose-based adsorbents for chromatography. Comparative study of adsorption capacities and desorption efficiencies.
Amphiphilic agarose-based adsorbents for chromatography. Comparative study of adsorption capacities and desorption efficiencies.
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用于色谱法的两亲性琼脂糖基吸附剂。
DOI：
10.1016/0021-9673(94)00857-6
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Porath,J
中科院分区：
文献类型：
--
作者：
Oscarsson,S;Angulo-Tatis,D;Chaga,G;Porath,J
研究了许多附着在交联琼脂糖上的疏水性衍生物作为蛋白质吸附剂。吸附和解吸行为的差异被确定为所选盐的类型和浓度的函数。辛基和苯基琼脂糖优先吸附血清白蛋白，而吡啶基-S-琼脂糖在高浓度易溶盐（例如硫酸盐）存在下对 IgG 显示出更强的优先亲和力。与吡啶基-S-琼脂糖相反，用 1 M NaOH 充分洗涤后，大部分蛋白质仍固定在辛基和苯基琼脂糖上。
A number of hydrophobic derivatives attached to cross-linked agarose were studied as protein adsorbents. Differences in the adsorption and desorption behaviour were determined as functions of type and concentration of selected salts. Whereas octyl- and phenyl-Sepharose adsorb serum albumin preferentially, pyridyl-S-agarose shows a much stronger preferential affinity for IgG in the presence of high concentrations of lyotropic salts, such as sulphates. In contrast to pyridyl-S-agarose, a large portion of proteins remained fixed to octyl- and phenyl-Sepharose after extensive washing with 1 M NaOH.