Using enzyme inhibition as a high throughput method to measure the enantiomeric excess of a chiral sulfoxide.
Using enzyme inhibition as a high throughput method to measure the enantiomeric excess of a chiral sulfoxide.
复制标题
使用酶抑制作为高通量方法来测量手性亚砜的对映体过量。
DOI：
10.1021/ol0521681
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发表时间：
2005
期刊：
影响因子：
5.2
通讯作者：
C. Seto
中科院分区：
文献类型：
--
作者：
C. Sprout;C. Seto
[反应：甲基对甲苯基亚砜的对映体过量可以通过测量其抑制醇脱氢酶催化的乙醇氧化的能力以高通量形式测定。亚砜的两种对映体对酶的抑制常数有很大的不同。因此，在亚砜存在下乙醇氧化的初始速率与亚砜对映体过量相关。
[reaction: see text] The enantiomeric excess of methyl p-tolyl sulfoxide can be determined in a high throughput format by measuring its ability to inhibit the alcohol dehydrogenase catalyzed oxidation of ethanol. The two enantiomers of the sulfoxide have very different inhibition constants for the enzyme. Thus, the initial rate of ethanol oxidation in the presence of the sulfoxide is correlated with the sulfoxide enantiomeric excess.