DIGE-based proteomic analysis identifies nucleophosmin/B23 and nucleolin C23 as over-expressed proteins in relapsed/refractory acute leukemia
DIGE-based proteomic analysis identifies nucleophosmin/B23 and nucleolin C23 as over-expressed proteins in relapsed/refractory acute leukemia
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基于 DIGE 的蛋白质组学分析确定核磷蛋白/B23 和核仁素 C23 是复发/难治性急性白血病中的过度表达蛋白
DOI：
10.1016/j.leukres.2011.01.010
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发表时间：
2011-08-01
影响因子：
2.7
通讯作者：
Chen, Yingyu
中科院分区：
文献类型：
--
作者：
Hu, Jianda;Lin, Minhui;Chen, Yingyu
耐药是急性白血病治疗中的一个难题。为了研究与耐药相关的新蛋白变化，基于蛋白质组学方法- 2d - dige和MALDI-TOF/MS比较了白血病细胞系HL-60和阿霉素耐药HL-60 （HL-60/ADR）之间的蛋白表达谱。HL-60/ADR中有13个蛋白点上调，3个蛋白点下调。筛选出核磷蛋白/B23 （NPM B23）和核蛋白C23 (C23)，经western blot验证，在白血病细胞中，特别是在3种耐药白血病细胞系和复发/难治患者中均有明显上调。综上所述，B23和C23可能参与了白血病的耐药过程，并可用于评估白血病的预后。(C) 2011 Elsevier Ltd.版权所有。
Drug resistance is a challenge in treatment of acute leukemia. To investigate novel protein changes involved in resistance, protein expression profiles between leukemia cell line HL-60 and adriamycin-resistant HL-60 (HL-60/ADR) were compared based on a proteomic approach-2D-DIGE followed by MALDI-TOF/MS. 13 protein spots were identified as up-regulated and 3 down-regulated in HL-60/ADR. Nucleophosmin/B23 (NPM B23) and nucleolin C23 (C23) were selected and verified by western blot, which showed an obvious up-regulation in leukemia cells, especially in 3 resistant leukemia cell lines and in relapsed/refractory patients. To a conclusion, B23 and C23 may be involved in drug resistance and be useful in assessing the prognosis of leukemia. (C) 2011 Elsevier Ltd. All rights reserved.