Design and biofabrication of dermal regeneration scaffolds: role of oligomeric collagen fibril density and architecture.
Design and biofabrication of dermal regeneration scaffolds: role of oligomeric collagen fibril density and architecture.
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DOI：
10.2217/rme-2019-0084
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发表时间：
2020-02
影响因子：
2.7
通讯作者：
David O. Sohutskay;Kevin P Buno;S. Tholpady;Samantha J Nier;S. Voytik-Harbin
中科院分区：
文献类型：
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作者：
David O. Sohutskay;Kevin P Buno;S. Tholpady;Samantha J Nier;S. Voytik-Harbin
目的：评价特定胶原纤维总含量和空间梯度的低聚胶原蛋白构建的真皮再生支架。材料和方法：通过电子显微镜和拉伸试验验证材料的微观结构和力学特征。测定了真皮支架诱导大鼠全层皮肤创面再生的能力，并与无填充对照、自体皮片和市售胶原敷料进行了比较。结果：低聚物支架中纤维含量的增加抑制了伤口的收缩，减少了肌成纤维细胞标志物的表达。在14天的时间里，支架的细胞和血管渗透随着纤维的密度和取向的不同而不同。结论：纤维含量、空间梯度和取向是胶原支架设计的重要考虑因素，可在减少伤口收缩的同时促进血管形成和真皮再生。
Aim: To evaluate dermal regeneration scaffolds custom-fabricated from fibril-forming oligomeric collagen where the total content and spatial gradient of collagen fibrils was specified. Materials & methods: Microstructural and mechanical features were verified by electron microscopy and tensile testing. The ability of dermal scaffolds to induce regeneration of rat full-thickness skin wounds was determined and compared with no fill control, autograft skin and a commercial collagen dressing. Results: Increasing fibril content of oligomer scaffolds inhibited wound contraction and decreased myofibroblast marker expression. Cellular and vascular infiltration of scaffolds over the 14-day period varied with the graded density and orientation of fibrils. Conclusion: Fibril content, spatial gradient and orientation are important collagen scaffold design considerations for promoting vascularization and dermal regeneration while reducing wound contraction.