Reappraisal of VAChT-Cre: Preference in slow motor neurons innervating type I or IIa muscle fibers
Reappraisal of VAChT-Cre: Preference in slow motor neurons innervating type I or IIa muscle fibers
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DOI：
10.1002/dvg.22979
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发表时间：
2016-11-01
期刊：
影响因子：
1.5
通讯作者：
Yamanaka, Tomoyuki
中科院分区：
文献类型：
--
作者：
Misawa, Hidemi;Inomata, Daijiro;Yamanaka, Tomoyuki
Vacht-CRE。FAST和Vacht-CRE。慢速小鼠出生后约有一半的体细胞运动神经元选择性表达Cre重组酶。这些小鼠品系已被用于对成年运动神经元进行选择性遗传修饰的各种研究。在本研究中，我们将Vacht-Cre系与报告系CAG-SYP/tdTomato杂交，其中突触素-tdTomato融合蛋白被有效地分离到轴突终末，从而使标记运动神经元的细胞体和轴突终末成为可能。在小鼠中，SYP/td番茄荧光优先与骨桥蛋白共定位，骨桥蛋白是最近发现的抗慢抽动疲劳(S)和快抽动抗疲劳(FR)类型的运动神经元标记物。该荧光不优先与快速抽动疲劳运动神经元的标志物--基质金属蛋白酶-9共定位。在神经肌肉接头处，SYP/tdTomato荧光主要分布在支配I型或IIa型肌纤维的运动神经末梢。这些结果表明，Vacht-CRE系是CRE驱动因子，对S和FR运动神经元具有选择性。为避免混淆，我们已将鼠标行名称从Vacht-CRE更改。FAST和Vacht-CRE。慢行至Vacht-CRE。早早和Vacht-CRE。分别是晚点。小鼠品系将成为研究与生理学和病理学有关的慢型运动神经元的有用工具。
VAChT-Cre. Fast and VAChT-Cre. Slow mice selectively express Cre recombinase in approximately one half of postnatal somatic motor neurons. The mouse lines have been used in various studies with selective genetic modifications in adult motor neurons. In the present study, we crossed VAChT-Cre lines with a reporter line, CAG-Syp/tdTomato, in which synaptophysin-tdTomato fusion proteins are efficiently sorted to axon terminals, making it possible to label both cell bodies and axon terminals of motor neurons. In the mice, Syp/tdTomato fluorescence preferentially co-localized with osteopontin, a recently discovered motor neuron marker for slow-twitch fatigue-resistant (S) and fast-twitch fatigue-resistant (FR) types. The fluorescence did not preferentially co-localize with matrix metalloproteinase-9, a marker for fast-twitch fatigable (FF) motor neurons. In the neuromuscular junctions, Syp/tdTomato fluorescence was detected mainly in motor nerve terminals that innervate type I or IIa muscle fibers. These results suggest that the VAChT-Cre lines are Cre-drivers that have selectivity in S and FR motor neurons. In order to avoid confusion, we have changed the mouse line names from VAChT-Cre. Fast and VAChT-Cre. Slow to VAChT-Cre. Early and VAChT-Cre. Late, respectively. The mouse lines will be useful tools to study slow-type motor neurons, in relation to physiology and pathology.