Enzymatic characterization of Aeromonas hydrophila complex by the API ZYM system

Enzymatic characterization of Aeromonas hydrophila complex by the API ZYM system
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通过 API ZYM 系统对嗜水气单胞菌复合体进行酶学表征

DOI:
10.1128/jcm.16.4.692-696.1982
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发表时间:
1982
影响因子:
9.4
通讯作者:
T. Hsu
T. Hsu
中科院分区:
医学2区
文献类型:
--
作者:
W. D. Waltman;E. Shotts;T. Hsu

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用API ZYM系统(Analytab Products,Plainview,NY)测定来自不同来源的48个嗜水气单胞菌复合体分离物的酶特性。所有分离株均缺乏缬氨酸和胱氨酸氨肽酶、胰凝乳蛋白酶、α-甘露糖苷酶、α-岩藻糖苷酶、α-半乳糖苷酶和β-葡萄糖醛酸酶,但具有辛酸酯酶-脂肪酶、亮氨酸氨肽酶、酸性磷酸酶、磷酰胺酶和N-乙酰基-β-葡萄糖苷酶。在碱性磷酸酶、丁酸酯酶、肉豆蔻酸脂酶、胰蛋白酶、β-半乳糖苷酶、α-葡萄糖苷酶和β-葡萄糖苷酶的存在下发现变异性。来自不同来源的菌株的酶谱之间没有显着差异。
Enzymatic characterization of 48 Aeromonas hydrophila complex isolates from various sources was determined with the API ZYM system (Analytab Products, Plainview, N.Y.). All isolates lacked valine and cystine aminopeptidases, chymotrypsin, alpha-mannosidase, alpha-fucosidase, alpha-galactosidase, and beta-glucuronidase but possessed caprylate esterase-lipase, leucine aminopeptidase, acid phosphatase, phosphoamidase, and N-acetyl-beta-glucosidase. Variability was found in the presence of alkaline phosphatase, butyrate esterase, myristate lipase, trypsin, beta-galactosidase, alpha-glucosidase, and beta-glucosidase. No significant differences were evident among the enzymatic profiles of isolates from various sources.