Real-time monitoring of polyacrylamide gel electrophoresis by schlieren optics.
Real-time monitoring of polyacrylamide gel electrophoresis by schlieren optics.
复制标题
通过纹影光学实时监测聚丙烯酰胺凝胶电泳。
DOI：
10.1093/oxfordjournals.jbchem.a122105
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
H. Kubota
中科院分区：
文献类型：
--
作者：
T. Takagi;P. Rao;H. Kubota
一个现代化的纹影光学应用于聚丙烯酰胺凝胶电泳过程中的蛋白质条带。可以检测到含有低至0.3微克蛋白质的条带。除了蛋白质条带，通常被忽视的现象，如缓冲液组分的边界迁移可以可视化。作为一个例子，这样得到的SDS-聚丙烯酰胺凝胶电泳与不连续缓冲液系统的电泳模式。发现分裂型彩色滤光片的使用对于根据折射率梯度的符号将凝胶上的特定位置着色为红色或蓝色是有用的。这种检测手段似乎也是有用的，因为，首先，它使电泳迁移率测量更定量，其次，它允许定位蛋白质条带并在完整状态下取样。
A modernized schlieren optics was applied to follow protein bands visually during polyacrylamide gel electrophoresis. A band containing as little as 0.3 microgram of a protein could be detected. Besides the protein bands, usually overlooked phenomena such as boundary migration of the buffer components could be visualized. As an example, electrophoretic patterns thus obtained for SDS-polyacrylamide gel electrophoresis with a discontinuous buffer system are presented. The use of a split-type colored filter was found to be useful for coloring a particular location on the gel red or blue depending on the sign of the refractive index gradient. This means of detection also seems useful in that, firstly, it makes electrophoretic mobility measurement more quantitative and, secondly, it allows localization of a protein band and sampling in the intact state.