Relationships between human cortical bone toughness and collagen cross-links on paired anatomical locations
Relationships between human cortical bone toughness and collagen cross-links on paired anatomical locations
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DOI：
10.1016/j.bone.2018.04.024
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发表时间：
2018-07-01
期刊：
影响因子：
4.1
通讯作者：
Mitton, David
中科院分区：
文献类型：
--
作者：
Gauthier, Remy;Follet, Helene;Mitton, David
人类皮质骨骨折过程取决于内部孔隙网络下至腔隙长度尺度。最近的研究结果表明，在胶原水平上，胶原交联的成熟可能对骨的力学行为产生负面影响。虽然已经研究了戊糖苷对人皮质骨韧性的影响，但仅研究了成熟和未成熟酶交联对强度和断裂功的影响。此外，这些关系还没有在不同的配对解剖位置上进行研究。因此，本研究的目的是评估酶和非酶胶原蛋白交联和人类皮质骨韧性之间的关系，在四个人类配对的解剖位置。单边缺口弯曲韧性测试进行了两个负载条件：准静态标准条件，和条件代表的下降。这些试验用32对股骨干、股骨颈和桡骨干进行（18名女性，年龄81 +/- 12岁; 14名男性，年龄79 +/- 8岁）。胶原酶和非酶交联测定相同的骨骼。胶原蛋白的成熟被定义为未成熟和成熟交联（CX）之间的比率。结果表明，当收集股骨和桡骨骨干时，胶原交联成熟与骨韧性之间存在显著相关性，但当单独考虑每个解剖位置时，这种相关性并不显著。这些结果表明，当考虑人皮质骨裂纹扩展机制时，胶原酶和非酶交联的影响很小。
Human cortical bone fracture processes depend on the internal porosity network down to the lacunar length scale. Recent results show that at the collagen scale, the maturation of collagen cross-links may have a negative influence on bone mechanical behavior. While the effect of pentosidine on human cortical bone toughness has been studied, the influence of mature and immature enzymatic cross-links has only been studied in relation to strength and work of fracture. Moreover, these relationships have not been studied on different paired anatomical locations. Thus, the aim of the current study was to assess the relationships between both enzymatic and non-enzymatic collagen cross-links and human cortical bone toughness, on four human paired anatomical locations. Single Edge Notched Bending toughness tests were performed for two loading conditions: a quasi-static standard condition, and a condition representative of a fall. These tests were done with 32 paired femoral diaphyses, femoral necks and radial diaphyses (18 women, age 81 +/- 12 y.o.; 14 men, age 79 +/- 8 y.o.). Collagen enzymatic and non-enzymatic crosslinks were measured on the same bones. Maturation of collagen was defined as the ratio between immature and mature cross-links (CX). The results show that there was a significant correlation between collagen cross-link maturation and bone toughness when gathering femoral and radial diaphyses, but not when considering each anatomical location individually. These results show that the influence of collagen enzymatic and non-enzymatic cross-links is minor when considering human cortical bone crack propagation mechanisms.