Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain
Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain
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DOI：
10.1016/j.mcn.2006.04.003
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发表时间：
2006-05-01
影响因子：
3.5
通讯作者：
Cremer, Harold
中科院分区：
文献类型：
--
作者：
Seidenfaden, Ralph;Desoeuvre, Angelique;Cremer, Harold
在成年小鼠的前脑中，大量的神经元前体在脑室下区（SVZ）产生，注定要成为产生GABA和多巴胺的嗅球（OB）中间神经元。尽管这种神经源性系统代表了脑修复方法中干细胞和祖细胞的潜在储存库，但关于svz来源的细胞在异位脑区域的存活和分化的信息仍然是零碎的。我们在这里表明，SVZ组织的异位移植产生了两种形态上可区分的细胞类型，显示出少突胶质细胞或星形胶质细胞的特征。由于SVZ组织含有神经元和胶质祖细胞，我们使用磁性细胞分选从分离的SVZ中去除A2135（+）胶质祖细胞，并积极选择表达PSA-NCAM的细胞。该程序允许纯化表达TUJ1、DCX和GAD65/67的神经元前体。这些细胞的移植再次导致了相同的两种胶质细胞类型的产生，这表明有行为的中间神经元前体在正常环境外经历了胶质细胞分化。(c) 2006爱思唯尔公司版权所有。
In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2135(+) glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment. (c) 2006 Elsevier Inc. All rights reserved.