Plant KASH proteins SINE1 and SINE2 have synergistic and antagonistic interactions with actin-branching and actin-bundling factors
Plant KASH proteins SINE1 and SINE2 have synergistic and antagonistic interactions with actin-branching and actin-bundling factors
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DOI：
10.1093/jxb/erad400
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发表时间：
2023-10-11
影响因子：
6.9
通讯作者：
Meier，Iris
中科院分区：
文献类型：
--
作者：
Moser，Morgan;Groves，Norman R.;Meier，Iris
核骨架和细胞骨架连接物（LINC）复合物由外核膜KASH蛋白组成，在核膜腔内与内核膜SUN蛋白相互作用，连接细胞核和细胞骨架。拟南芥KASH蛋白SINE1和SINE2在光暗转换和脱落酸（ABA）诱导的气孔动力学中起作用，这需要F-actin重组。SINE2影响肌动蛋白解聚和SINE1影响肌动蛋白再聚合。肌动蛋白相关蛋白2/3 （ARP2/3）复合体、肌动蛋白成核器和植物肌动蛋白捆绑和稳定因子SCAB1参与气孔孔径的调控。在这里，我们测试了SINE1和SINE2与SCAB1和ARP2/3复合物的遗传相互作用。我们发现，在aba诱导的气孔关闭过程中，SINE1和ARP2/3复合体在同一途径中发挥作用，而SINE2和ARP2/3复合体发挥相反的作用。肌动蛋白重聚合缺陷在sine1- 2 - sine1-1中被部分修复，而sine2则上位于sine1。此外，SINE1和ARP2/3在侧根发育中协同作用。SINE2的缺失使得毛状体的发育不依赖于ARP2/3复合体。总之，这些数据揭示了两种KASH蛋白与肌动蛋白重塑装置之间复杂而不同的相互作用，并为SINE1和SINE2在肌动蛋白动力学中的差异作用提供了证据。
Linker of nucleoskeleton and cytoskeleton (LINC) complexes consist of outer nuclear membrane KASH proteins, interacting in the nuclear envelope lumen with inner nuclear membrane SUN proteins and connecting the nucleus and cytoskeleton. The paralogous Arabidopsis KASH proteins SINE1 and SINE2 function during stomatal dynamics induced by light–dark transitions and abscisic acid (ABA), which requires F-actin reorganization. SINE2 influences actin depolymerization and SINE1 actin repolymerization. The actin-related protein 2/3 (ARP2/3) complex, an actin nucleator, and the plant actin-bundling and -stabilizing factor SCAB1 are involved in stomatal aperture control. Here, we have tested the genetic interaction of SINE1 and SINE2 with SCAB1 and the ARP2/3 complex. We show that SINE1 and the ARP2/3 complex function in the same pathway during ABA-induced stomatal closure, while SINE2 and the ARP2/3 complex play opposing roles. The actin repolymerization defect observed insine1-1is partially rescued inscab1-2 sine1-1, whileSINE2is epistatic toSCAB1. In addition, SINE1 and ARP2/3 act synergistically in lateral root development. The absence of SINE2 renders trichome development independent of the ARP2/3 complex. Together, these data reveal complex and differential interactions of the two KASH proteins with the actin-remodeling apparatus and add evidence to the proposed differential role of SINE1 and SINE2 in actin dynamics.