Metalloproteinases in endochondral bone formation: appearance of tissue inhibitor-resistant metalloproteinases.
Metalloproteinases in endochondral bone formation: appearance of tissue inhibitor-resistant metalloproteinases.
复制标题
软骨内骨形成中的金属蛋白酶：组织抑制剂抗性金属蛋白酶的出现。
DOI：
10.1016/0003-9861(87)90524-8
复制
发表时间：
1987
影响因子：
3.9
通讯作者：
Cheng,YS
中科院分区：
文献类型：
--
作者：
Mikuni-Takagaki,Y;Cheng,YS
解剖的胚胎鸡肢体在软骨内骨发育过程中释放中性金属蛋白酶。这些酶降解培养基中的软骨蛋白聚糖和明胶。我们发现酶在由邻近骨髓腔（腔周围）区域的外植体调节的培养基中具有活性。这些酶降解蛋白聚糖 (PG) 和/或明胶。这些自发活性酶对血清和组织蛋白酶抑制剂、α2-巨球蛋白和软骨金属蛋白酶抑制剂 (TIMP) 具有抵抗力。从跗节和骨髓外植体分泌的其他酶大多潜伏在培养基中。活化的跗节酶（PG 降解酶和明胶分解酶）被上述抑制剂阻断。活化的骨髓酶不会降解 PG，但对这些抑制剂有抵抗力。由于空腔周围酶对组织和血清抑制剂具有抵抗力，因此它们可能在长骨胚胎成骨中发挥重要作用。空腔周围酶被激活的体内机制尚未确定。
Dissected embryonic chick limbs release neutral metalloproteinases during endochondral bone development. These enzymes degrade cartilage proteoglycan and gelatin in culture medium. We found the enzymes active in the medium conditioned by explants of the region adjacent to the bone marrow cavity (cavity-surround). These enzymes degrade proteoglycan (PG) and/or gelatin. These spontaneously active enzymes are resistant to serum and tissue proteinase inhibitors,α2-macroglobulin, and cartilage metalloproteinase inhibitor (TIMP). The other enzymes secreted from tarsus and bone marrow explants are mostly latent in the culture medium. Activated tarsus enzymes (PG degrading and gelatinolytic) are blocked by the above inhibitors. Activated marrow enzyme does not degrade PG but is resistant to those inhibitors. Cavity-surround enzymes may play an important role in embryonic osteogenesis of long bones because of their resistance to tissue and serum inhibitors. Thein vivomechanisms by which cavity-surround enzymes are activated are yet to be determined.