Chloroplast proteomics: potentials and challenges
Chloroplast proteomics: potentials and challenges
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DOI：
10.1093/jxb/erh104
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发表时间：
2004-05-01
影响因子：
6.9
通讯作者：
Gruissem, W
中科院分区：
文献类型：
--
作者：
Baginsky, S;Gruissem, W
随着拟南芥基因组的问世和水稻基因组测序计划的接近完成，大规模的植物蛋白质质谱分析已经成为可能。确定细胞的蛋白质组是一项具有挑战性的任务，它被蛋白质组动力学和复杂性所复杂化。蛋白质的生物化学异质性限制了标准化分析方法的使用，并要求蛋白质组分析使用苛刻的技术。植物细胞器的蛋白质组学研究已有报道，包括叶绿体和线粒体。植物生物学家对叶绿体特别感兴趣，因为叶绿体具有复杂的生化途径，具有重要的代谢功能。因此，来自叶绿体蛋白质组的信息将为途径区隔和蛋白质分类提供新的见解。本文讨论了叶绿体蛋白质组分析的一些方法和叶绿体蛋白质组研究的前景。
With the available Arabidopsis genome and near-completion of the rice genome sequencing project, large-scale analysis of plant proteins with mass spectrometry has now become possible. Determining the proteome of a cell is a challenging task, which is complicated by proteome dynamics and complexity. The biochemical heterogeneity of proteins constrains the use of standardized analytical procedures and requires demanding techniques for proteome analysis. Several proteome studies of plant cell organelles have been reported, including chloroplasts and mitochondria. Chloroplasts are of particular interest for plant biologists because of their complex biochemical pathways for essential metabolic functions. Information from the chloroplast proteome will therefore provide new insights into pathway compartmentalization and protein sorting. Some approaches for the analysis of the chloroplast proteome and future prospects of plastid proteome research are discussed here.