Water Sorption in Pretreated Grasses as a Predictor of Enzymatic Hydrolysis Yields
Water Sorption in Pretreated Grasses as a Predictor of Enzymatic Hydrolysis Yields
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预处理草中的水吸附作为酶水解产量的预测因子
DOI：
10.1016/j.biortech.2017.08.200
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发表时间：
2017
影响因子：
11.4
通讯作者：
Hodge, David B.
中科院分区：
文献类型：
--
作者：
Williams, Daniel L.;Crowe, Jacob D.;Ong, Rebecca G.;Hodge, David B.
研究了氨纤维膨胀（AFEX）和碱性过氧化氢（AHP）脱木质素在一定条件下对玉米秸秆和柳枝稷性能的影响。预处理引起的原料性质的变化随后与酶水解产率进行比较，以检查酶水解与细胞壁性质之间的关系。预处理通过不同的机制提高酶解产率；AFEX预处理通过木质素再定位和部分木聚糖增溶，AHP主要通过木质素增溶。这项工作的一个重要结果表明，虽然ahp脱木质素生物质中木质素含量的变化与水解反应的改善明显相关，但单靠afex预处理生物质中木质素含量的变化并不能解释水解率的差异。我们确定了水保留值，它表征了水与预处理生物质细胞壁的关系，可以用来预测本研究中所有预处理生物质的水解产率。
This work investigated the impact of two alkaline pretreatments, ammonia fiber expansion (AFEX) and alkaline hydrogen peroxide (AHP) delignification performed over a range of conditions on the properties of corn stover and switchgrass. Changes in feedstock properties resulting from pretreatment were subsequently compared to enzymatic hydrolysis yields to examine the relationship between enzymatic hydrolysis and cell wall properties. The pretreatments function to increase enzymatic hydrolysis yields through different mechanisms; AFEX pretreatment through lignin relocalization and some xylan solubilization and AHP primarily through lignin solubilization. An important outcome of this work demonstrated that while changes in lignin content in AHP-delignified biomass could be clearly correlated to improved response to hydrolysis, compositional changes alone in AFEX-pretreated biomass could not explain differences in hydrolysis yields. We determined the water retention value, which characterizes the association of water with the cell wall of the pretreated biomass, can be used to predict hydrolysis yields for all pretreated biomass within this study.