Monoclonal antibody-based fluorescence polarization immunoassay for high throughput screening of furaltadone and its metabolite AMOZ in animal feeds and tissues.
Monoclonal antibody-based fluorescence polarization immunoassay for high throughput screening of furaltadone and its metabolite AMOZ in animal feeds and tissues.
复制标题
DOI：
10.2174/1386207311316060010
复制
发表时间：
2013-06
影响因子：
1.8
通讯作者：
Zhenlin Xu;Shiwei Zhang;Yuan-ming Sun;Yu-Dong Shen;Hongtao Lei;Yue-Ming Jiang;S. Eremin;Jinyi Yang;Hong Wang
中科院分区：
文献类型：
--
作者：
Zhenlin Xu;Shiwei Zhang;Yuan-ming Sun;Yu-Dong Shen;Hongtao Lei;Yue-Ming Jiang;S. Eremin;Jinyi Yang;Hong Wang
开发了一种简单快速的均相荧光偏振免疫分析 (FPIA)，用于测定呋喃他酮及其代谢物 3-氨基-5-甲基吗啉代-2-恶唑烷酮 (AMOZ)。针对一种新型免疫原制备了与呋喃他酮和 AMOZ 硝基苯基衍生物 (NPAMOZ) 具有高交叉反应性的单克隆抗体，并研究了几种合成示踪剂对 FPIA 敏感性的影响。所提出的 FPIA 使用最佳抗体和示踪剂对，呋喃他酮的 IC50 为 4.3 µg L-1，检测限为 0.6 µg L-1，NPAMOZ 的检测限为 2.7 µgL-1 和 0.3 µg L-1。 FPIA 从动物饲料中回收呋喃他酮的范围为 79.6% 至 87.7%，而从动物组织中回收 AMOZ 的回收率为 72.9% 至 83.1%。 FPIA 的结果与标准分析方法的结果之间具有良好的相关性（R>0.99）。 FPIA 不需要分离或洗涤步骤，抗体-示踪剂相互作用平衡所需的总时间仅为 10 分钟。这些结果表明，所提出的 FPIA 在高通量筛选动物饲料中呋喃他酮残留及其在动物组织中代谢物 AMOZ 残留方面具有巨大的潜力和实用性。
A simple and fast homogeneous fluorescent polarization immunoassay (FPIA) was developed for the determination of furaltadone and its metabolite 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ). Monoclonal antibody with high cross-reactivity to furaltadone and the nitrophenyl derivative of AMOZ (NPAMOZ) were produced against a novel immunogen and the effects of several synthesized tracers on FPIA sensitivity studied. The proposed FPIA, using an optimum antibody and tracer pair, had an IC₅₀ of 4.3 µg L⁻¹ and limit of detection at 0.6 µg L⁻¹ for furaltadone, and 2.7 µgL⁻¹ and 0.3 µg L⁻¹ for NPAMOZ. Recoveries of furaltadone from animal feeds by FPIA ranged from 79.6 to 87.7%, while recoveries of AMOZ from animal tissues ranged from 72.9 to 83.1%. Good correlation (R>0.99) between the results of this FPIA and a standard analytical method was obtained. The FPIA does not require separation or washing steps and the total time required for equilibrium of the antibody-tracer interaction is only 10 min. These results indicated that the proposed FPIA offers great potential and utility for the high throughput screening of furaltadone residues in animal feed and its metabolite AMOZ residues in animal tissues.