Over 1000-fold improvement in an online preconcentration of trace anionic compounds by capillary electrophoresis with ionic liquid micelle-based three-step stacking
Over 1000-fold improvement in an online preconcentration of trace anionic compounds by capillary electrophoresis with ionic liquid micelle-based three-step stacking
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使用基于离子液体胶束的三步堆积毛细管电泳将痕量阴离子化合物的在线预浓缩提高了 1000 倍以上
DOI：
10.1016/j.aca.2018.08.027
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发表时间：
2018
影响因子：
6.2
通讯作者：
魏蒙蒙
中科院分区：
文献类型：
--
作者：
楚楚;魏蒙蒙
开发了一种灵敏、简单、环保的在线三步叠加策略，结合现场增强样品注入、扫描和胶束到溶剂的叠加，用于测定复杂生物样品中的痕量有机阴离子分析物。一种绿色离子液体，1-十二烷基-3-甲基咪唑硫酸氢，首次作为胶束溶液被引入，并用于电渗透回流的堆叠策略。讨论了堆积机理，优化了影响三步堆积富集效率的参数。离子液体包膜毛细管制备简单、可再生、重复性好（RSD<2.16%），效率高。在最优条件下，与普通毛细管区带电泳模式相比，灵敏度提高了2424倍。与传统毛细管区带电泳相比，模型碱性有机酸的峰形和分离效率有显著提高。优化后的方法对复杂基质中痕量浓度分析物的定量分析具有很大的潜力。
A sensitive, simple, and environmentally friendly online three-step stacking strategy combining field-enhanced sample injection, sweeping and micelle to solvent stacking has been developed to determine trace amounts of organic anionic analytes in complex biological samples. A green ionic liquid, 1-dodecyl-3-methylimidazolium hydrogen sulfate, was first introduced as a micellar solution and used for electroosmotic flow reversal in this stacking strategy. The mechanism of stacking has been discussed, and parameters affecting three-step stacking preconcentration efficiency have been optimized. The capillary coated with an ionic liquid was easy to prepare, regenerable and repeatable (RSD<2.16%) and yielded a high efficiency. Under optimal conditions, the sensitivity improvement increased up to 2424-fold when compared with the normal capillary zone electrophoresis mode. The peak shape and separation efficiency of the model basic organic acids showed a significant enhancement when compared to traditional capillary zone electrophoresis. The optimized method showed great potential for quantitative analysis of trace concentration analytes in complex matrices.