Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid.
Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid.
复制标题
DOI：
10.1021/bm200983k
复制
发表时间：
2011-11-14
影响因子：
6.2
通讯作者：
Finn MG
中科院分区：
文献类型：
--
作者：
Rhee JK;Hovlid M;Fiedler JD;Brown SD;Manzenrieder F;Kitagishi H;Nycholat C;Paulson JC;Finn MG
使用通过特定工程RNA-蛋白质相互作用增强的模块化系统，以高产率产生包封多个拷贝的荧光蛋白的Qβ病毒样颗粒。所得颗粒在结构上与单独的重组Qβ无区别。的crossidated蛋白质几乎相同的单体类似物的光化学性质，更稳定的热降解，并保护蛋白水解裂解。外衣壳表面上的残基通过酰化和叠氮化物-炔环加成进行化学衍生化，而不影响包装蛋白的荧光性质。一个高亲和力的碳水化合物为基础的配体的CD 22受体，从而连接，并通过流式细胞仪和激光共聚焦显微镜成功地检测到特定的细胞标记的颗粒。
Qβ virus-like particles encapsulating multiple copies of fluorescent proteins were generated in high yields using a modular system enhanced by specific engineered RNA-protein interactions. The resulting particles were structurally indistinguishable from recombinant Qβ alone. The encapsidated proteins were nearly identical in photochemical properties to monomeric analogues, were more stable toward thermal degradation, and were protected from proteolytic cleavage. Residues on the outer capsid surface were chemically derivatized by acylation and azide-alkyne cycloaddition without affecting the fluorescence properties of the packaged proteins. A high affinity carbohydrate-based ligand of the CD22 receptor was thereby attached, and specific cell labeling by the particles was successfully detected by flow cytometry and confocal laser microscopy.
登录
查看更多内容
影响因子：
15
作者：
Prasuhn, Duane E., Jr.;Singh, Pratik;Finn, M. G.
通讯作者：
Finn, M. G.
影响因子：
15
作者：
Hooker, JM;Kovacs, EW;Francis, MB
通讯作者：
Francis, MB
影响因子：
46.9
作者：
Pédelacq, JD;Cabantous, S;Waldo, GS
通讯作者：
Waldo, GS
DOI：
10.1073/pnas.0500929102
发表时间：
2005-03-22
影响因子：
11.1
作者：
Kickhoefer, VA;Garcia, Y;Rome, LH
通讯作者：
Rome, LH
影响因子：
4.6
作者：
Minten, Inge J.;Nolte, Roeland J. M.;Cornelissen, Jeroen J. L. M.
通讯作者：
Cornelissen, Jeroen J. L. M.