Proteomic analysis of the enzymes involved in the starch biosynthesis of maize with different endosperm type and characterization of the starch
Proteomic analysis of the enzymes involved in the starch biosynthesis of maize with different endosperm type and characterization of the starch
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DOI：
10.1002/jsfa.6054
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发表时间：
2013-08-30
影响因子：
4.1
通讯作者：
Arturo Bello-Perez, Luis
中科院分区：
文献类型：
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作者：
Juarez-Garcia, Erika;Agama-Acevedo, Edith;Arturo Bello-Perez, Luis
背景对不同胚乳类型的淀粉玉米进行了表征，并在授粉后20天和50天（DAP）对其生物合成酶进行了蛋白质组学分析。结果两种胚乳类型在20DAP时存在差异，主要在淀粉积累、直链淀粉含量、颗粒大小和结晶度百分比方面，而在50DAP时发现的差异在淀粉含量、颗粒大小、链的情况下不相关。长度分布和热性能。鉴定出 SSSI、SBEIIb 和 GBSSI 酶；然而，SBEIIb 仅在两个样品中被鉴定：20 DAP 的粉质胚乳和 50 DAP 的玻璃质胚乳。 结论 在达到成熟时，两个胚乳中的淀粉在形态或结构特征上没有显示出差异。通过基质辅助激光解吸/电离飞行时间鉴定的淀粉生物合成酶并未显示出与淀粉结构的关系。 (c) 2013年化学工业学会
BACKGROUNDThe characterization of starch maize with different endosperm type and the proteomic analysis of its biosynthetic enzymes at 20 and 50 days after pollination (DAP) was carried out.RESULTSThere were differences between both endosperm types at 20 DAP, mainly in starch accumulation, amylose content, granule size and crystallinity percentage, whereas at 50 DAP the differences found were not relevant in the case of starch content, granule size, chain length distribution and thermal properties. SSSI, SBEIIb and GBSSI enzymes were identified; however, SBEIIb was only identified in two samples: floury endosperm at 20 DAP and vitreous at 50 DAP.ConclusionStarch did not show differences in its morphological or structural characteristics in either endosperm on reaching maturity. Starch biosynthetic enzymes identified by matrix-assisted laser desorption/ionization-time of flight did not show a relationship to starch structure. (c) 2013 Society of Chemical Industry