Deformed wing virus: replication and viral load in mites (Varroa destructor)
Deformed wing virus: replication and viral load in mites (Varroa destructor)
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DOI：
10.1099/vir.0.005579-0
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发表时间：
2009-02-01
影响因子：
3.8
通讯作者：
Genersch, Elke
中科院分区：
文献类型：
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作者：
Gisder, Sebastian;Aumeier, Pia;Genersch, Elke
变形翼病毒（DWV）通常引起隐蔽感染，但当由外寄生螨瓦螨（Varroa destructor）传播时，可通过诱导形态畸形甚至死亡对蜜蜂产生破坏性影响。为了确定毁灭弧菌在残翅发育中的作用，我们分析了个体螨对DWV的存在和复制。结果支持螨虫病毒复制与形态畸形蜜蜂之间的相关性。病毒基因组等价物的量化显示，能够诱导明显DWV感染的螨每只螨含有10(10)-10（12）个基因组等价物。相比之下，不能诱导残翅的螨虫每只最多只含有10(8)个病毒基因组当量。结果表明，残翅的发育不仅取决于消弱弧菌对DWV的传播，还与消弱弧菌体内的病毒复制和寄生螨体内的DWV滴度有关。
Deformed wing virus (DWV) normally causes covert infections but can have devastating effects on bees by inducing morphological deformity or even death when transmitted by the ectoparasitic mite Varroa destructor. In order to determine the role of V. destructor in the development of crippled wings, we analysed individual mites for the presence and replication of DWV. The results supported the correlation between viral replication in mites and morphologically deformed bees. Quantification of viral genome equivalents revealed that mites capable of inducing an overt DWV infection contained 10(10)-10(12) genome equivalents per mite. In contrast, mites which could not induce crippled wings contained a maximum of only 10(8) viral genome equivalents per mite. We conclude that the development of crippled wings not only depends on DWV transmission by V. destructor but also on viral replication in V. destructor and on the DWV titre in the parasitizing mites.