Pharyngeal Arch Deficiencies Affect Taste Bud Development in the Circumvallate Papilla With Aberrant Glossopharyngeal Nerve Formation
Pharyngeal Arch Deficiencies Affect Taste Bud Development in the Circumvallate Papilla With Aberrant Glossopharyngeal Nerve Formation
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咽弓缺陷影响周围乳头味蕾发育并伴有舌咽神经形成异常
DOI：
10.1002/dvdy.24289
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发表时间：
2015
影响因子：
2.5
通讯作者：
Shinji Takada
中科院分区：
文献类型：
--
作者：
Tadashi Okubo;Shinji Takada
咽弓（pharyngeal arches，PA）是包括舌在内的颅部器官的发育器官。在胚胎舌表面的特定位置形成的味基板分化成位于不同味觉乳头中的味蕾。发育中的舌也有一个复杂的脑神经供应通过每个PA。结果Riparin 3纯合子突变小鼠第3、第4嗅区受损后，尽管味觉基板正常形成，但其轮廓乳头发育不全，味蕾缺失。在E12.5时，Riparin 3突变体胚胎的舌咽神经节的形成是有缺陷的，并且朝向舌后部的神经支配完全缺失。此外，在E9.5-E10.5的Riparin 3突变体胚胎的第三PA区域，来自上鳃基板的神经母细胞的分布严重但不完全地减弱，神经嵴细胞减少。在Tbx 1纯合子突变胚胎中，表现出另一种类型的PA发育缺陷，观察到发育不全的轮廓乳头沿着舌咽神经节的异常形成和严重受损的神经支配。结论PA缺陷影响味蕾发育的多个方面，包括颅神经节的形成和后舌的神经支配。发育动力学244：874-887，2015。© 2015 Wiley Periodicals，Inc.
BackgroundThe pharyngeal arches (PAs) generate cranial organs including the tongue. The taste placodes, formed in particular locations on the embryonic tongue surface, differentiate into taste buds harbored in distinct gustatory papillae. The developing tongue also has a complex supply of cranial nerves through each PA. However, the relationship between the PAs and taste bud development is not fully understood.ResultsRipply3homozygous mutant mice, which have impaired third/fourth PAs, display a hypoplastic circumvallate papilla and lack taste buds, although the taste placode is normally formed. Formation of the glossopharyngeal ganglia is defective and innervation toward the posterior tongue is completely missing inRipply3mutant embryos at E12.5. Moreover, the distribution of neuroblasts derived from the epibranchial placode is severely, but not completely, atenuated, and the neural crest cells are diminished in the third PA region ofRipply3mutant embryos at E9.5–E10.5. InTbx1homozygous mutant embryos, which exhibit another type of deficiency in PA development, the hypoplastic circumvallate papilla is observed along with abnormal formation of the glossopharyngeal ganglia and severely impaired innervation.ConclusionsPA deficiencies affect multiple aspects of taste bud development, including formation of the cranial ganglia and innervation to the posterior tongue.Developmental Dynamics 244:874–887, 2015. © 2015 Wiley Periodicals, Inc.