Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells
Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells
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DOI：
10.1016/j.tox.2005.05.007
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发表时间：
2005-09-15
期刊：
影响因子：
4.5
通讯作者：
Jan, KY
中科院分区：
文献类型：
--
作者：
Gurr, JR;Wang, ASS;Jan, KY
超细二氧化钛（TiO 2）颗粒暴露于UVA辐射时显示出强烈的细胞毒性，但在没有光活化的情况下被认为是一种生物相容性材料。与此概念相反，本研究结果表明，锐钛矿大小（10和20 nm）的二氧化钛颗粒在光活化诱导的氧化DNA损伤，脂质过氧化和微核形成的情况下，并增加过氧化氢和一氧化氮的生产BEAS-2B细胞，人支气管上皮细胞系。然而，在没有光照射的情况下，用锐钛矿尺寸（200和> 200 nm）的颗粒处理不会诱导氧化应激;似乎颗粒越小，颗粒越容易诱导氧化损伤。报道了金红石型TiO 2的光催化活性高于金红石型。与此相反，本研究结果表明，金红石大小的200 nm的颗粒诱导过氧化氢和氧化DNA损伤在没有光的情况下，但锐钛矿大小的200 nm的颗粒没有。在完全黑暗中，使用锐钛矿-金红石混合物的处理比单独使用锐钛矿或金红石形式的处理也检测到稍高水平的氧化DNA损伤。这些结果表明，动脉内滴注超细TiO 2颗粒可能会引起炎症反应。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Ultrafine titanium dioxide (TiO2) particles have been shown to exhibit strong cytotoxicity when exposed to UVA radiation, but are regarded as a biocompatible material in the absence of photoactivation. In contrast to this concept, the present results indicate that anatase-sized (10 and 20 nm) TiO2 particles in the absence of photoactivation induced oxidative DNA damage, lipid peroxidation, and micronuclei formation, and increased hydrogen peroxide and nitric oxide production in BEAS-2B cells, a human bronchial epithelial cell line. However, the treatment with anatase-sized (200 and > 200 nm) particles did not induce oxidative stress in the absence of light irradiation; it seems that the smaller the particle, the easier it is for the particle to induce oxidative damage. The photocatalytic activity of the anatase form of TiO2 was reported to be higher than that of the rutile form. In contrast to this notion, the present results indicate that rutile-sized 200 nm particles induced hydrogen peroxide and oxidative DNA damage in the absence of light but the anatase-sized 200 nm particles did not. In total darkness, a slightly higher level of oxidative DNA damage was also detected with treatment using an anatase-rutile mixture than with treatment using either the anatase or rutile forms alone. These results suggest that intratracheal instillation of ultrafine TiO2 particles may cause an inflammatory response. (c) 2005 Elsevier Ireland Ltd. All rights reserved.