Characterization of the ALP1 gene locus of Trichophyton tonsurans.
Characterization of the ALP1 gene locus of Trichophyton tonsurans.
复制标题
断发毛癣菌 ALP1 基因座的表征。
DOI：
10.1007/s11046-005-0132-0
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发表时间：
2005
期刊：
影响因子：
5.5
通讯作者：
Abdel-Rahman,SusanM
中科院分区：
文献类型：
--
作者：
Bhathena,Anahita;Gaedigk,Roger;Abdel-Rahman,SusanM
断端毛癣菌是儿科人群真菌感染的主要病原体。建立有利于菌株鉴别的技术提供了研究真菌基因型、生化表型和宿主疾病表现之间关系的机会。在进一步研究断苏氏锥虫种内遗传变异的过程中，我们发现了ALP 1基因的2个遗传多态性：5 'UTR的片段长度多态性和3' UTR的单核苷酸多态性（G→A）。全序列数据显示，长度多态性是由一个16 bp的重复元件，存在于串联从3到6倍，这取决于菌株。定量RT-PCR分析表明，长度多态性和ALP 1 mRNA转录水平之间存在明显的关联。这项研究中发现的序列变异不仅增加了我们区分T的能力。tonsuransstrains，但他们也揭示了存在的遗传变异与功能的后果，在转录水平，可能发挥作用，在调节疾病的严重程度。
Trichophyton tonsuransis the primary etiologic agent of fungal infections in the pediatric population. Establishing techniques that facilitate strain discrimination offer the opportunity to investigate the relationship between fungal genotype, biochemical phenotype and disease presentation in the host. In the process of expanding efforts to elucidate intra-specific genetic variability inT. tonsurans, we have identified 2 genetic polymorphisms in theALP1gene: a fragment length polymorphism in the 5’UTR and a single SNP (G→A) within the 3′UTR. Full sequence data revealed that the length polymorphism was constituted by a 16 bp repeat element, present in tandem from 3 to 6 times depending on the strain. Quantitative RT-PCR analysis demonstrated a clear association between the length polymorphism and ALP1 mRNA transcript levels. Not only do the sequence variations identified in this study increase our ability to discriminateT. tonsuransstrains, but they also reveal the presence of a genetic variation with functional consequences at the transcript level that may play a role in regulating disease severity.