Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei.
Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei.
复制标题
布氏锥虫转化过程中糖蛋白合成的抑制和表面涂层的释放。
DOI：
10.1002/j.1460-2075.1983.tb01648.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
C. Nonnengaesser
中科院分区：
文献类型：
--
作者：
P. Overath;J. Czichos;U. Stock;C. Nonnengaesser
本文研究了布氏锥虫427变异克隆MITat 1.4（117）在体外由血流型锥鞭毛体转化为前环型锥鞭毛体过程中变异表面糖蛋白（VSG）的生物合成及其从表面的释放。在27 ℃下转移到转化培养基后，VSG合成被抑制了一半时间，t1/2 = 30 min。同时VSG特异性mRNA丢失，表明抑制作用在转录水平上起作用。编码VSG的表达连锁的额外基因拷贝在转化期间和完成后保留。在VSG合成阻遏后，表面VSG从细胞脱落到培养基中。在释放部分VSG（表观摩尔数）时，重量61 000）被蛋白水解裂解为产物（表观摩尔数）。重量51 000），其代表蛋白质的N末端结构域，如通过不存在通常连接至C末端的碳水化合物部分来判断。
The biosynthesis of the variant surface glycoprotein (VSG) and its release from the surface of Trypanosoma brucei 427 variant clone MITat 1.4 (117) during in vitro transformation of bloodstream trypomastigotes to procyclic trypomastigotes was investigated. After transfer to the transformation medium at 27 degrees C, VSG synthesis is repressed with a half‐time, t1/2 = 30 min. Concomitantly VSG‐specific mRNA is lost suggesting that repression operates at the transcriptional level. The expression‐linked extra gene copy, which codes for VSG, is retained during and after completion of transformation. After repression of VSG synthesis, surface VSG is shed from the cells into the culture medium. During release part of VSG (apparent mol. wt. 61 000) is proteolytically cleaved to a product (apparent mol. wt. 51 000) which represents the N‐terminal domain of the protein as judged by the absence of the carbohydrate moiety normally linked to the C terminus.