Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses
Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses
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DOI：
10.1126/science.280.5370.1763
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发表时间：
1998-06-12
期刊：
影响因子：
56.9
通讯作者：
Pozzan, T
中科院分区：
文献类型：
--
作者：
Rizzuto, R;Pinton, P;Pozzan, T
用两种不同颜色、特异性靶向的绿色荧光蛋白，在三维高分辨率下分析了活体HeLa细胞线粒体和内质网(ER)之间的空间关系。观察到这些细胞器之间有许多紧密的接触，线粒体在原位形成了一个基本上相互连接的动态网络。一个定位于线粒体内膜外表面的钙敏感光蛋白显示，当内质网的三磷酸肌醇(IP3)门控通道开放时，线粒体表面暴露于比整体胞浆更高浓度的钙。这些结果强调了细胞结构和细胞器分布在钙信号调节中的重要性。
The spatial relation between mitochondria and endoplasmic reticulum (ER) in living HeLa cells was analyzed at high resolution in three dimensions with two differently colored, specifically targeted green fluorescent proteins. Numerous close contacts were observed between these organelles, and mitochondria in situ formed a largely interconnected, dynamic network. A Ca2+-sensitive photoprotein targeted to the outer face of the inner mitochondrial membrane showed that, upon opening of the inositol 1,4,5-triphosphate (IP3)-gated channels of the ER, the mitochondrial surface was exposed to a higher concentration of Ca2+ than was the bulk cytosol. These results emphasize the importance of cell architecture and the distribution of organelles in regulation of Ca2+ signaling.